Growing environmental concern sparks renewed interest in the sustainable production of (bio)materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are isotactic polymers that play a critical role in the central metabolism of producer bacteria, as they act as dynamic reservoirs of carbon and reducing equivalents. PHAs continue to attract industrial attention as a starting point toward renewable, biodegradable, biocompatible, and versatile thermoplastic and elastomeric materials. Pseudomonas species have been known for long as efficient biopolymer producers, especially for medium‐chain‐length PHAs. The surge of synthetic biology and metabolic engineering approaches in recent years offers the possibility of exploiting the untapped potential of Pseudomonas cell factories for the production of tailored PHAs. In this article, an overview of the metabolic and regulatory circuits that rule PHA accumulation in Pseudomonas putida is provided, and approaches leading to the biosynthesis of novel polymers (e.g., PHAs including nonbiological chemical elements in their structures) are discussed. The potential of novel PHAs to disrupt existing and future market segments is closer to realization than ever before. The review is concluded by pinpointing challenges that currently hinder the wide adoption of bio‐based PHAs, and strategies toward programmable polymer biosynthesis from alternative substrates in engineered P. putida strains are proposed.

中文翻译：

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工程恶臭假单胞菌的天然和合成途径，用于生产量身定制的聚羟基链烷酸酯

对环境的日益关注引发了人们对可替代石油衍生产品的（生物）材料可持续生产的新兴趣。聚羟基链烷酸酯（PHA）是全同立构聚合物，在生产菌的中央代谢中起着关键作用，因为它们充当了动态的碳库并减少了当量。作为可再生，可生物降解，生物相容以及通用的热塑性和弹性体材料的起点，PHA一直吸引着工业关注。假单胞菌菌种一直以来都是有效的生物聚合物生产者，尤其是中链长度的PHA。近年来合成生物学和代谢工程方法的激增提供了利用假单胞菌尚未开发的潜力的可能性电池工厂，用于量身定制的PHA。在本文中，概述了控制恶臭假单胞菌中PHA积累的代谢和调节回路，并讨论了导致新型聚合物（例如，在其结构中包含非生物化学元素的PHA）生物合成的方法。新颖的PHA破坏现有和未来市场细分市场的潜力比以往任何时候都更加接近实现。总结通过指出当前阻碍生物基PHA广泛采用的挑战来结束，并提出了从恶臭假单胞菌菌株中通过替代底物实现可编程聚合物生物合成的策略。