Reactive oxygen species‐responsive silk sericin microcapsules used for antioxidative stress damage,Microscopy Research and Technique

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Reactive oxygen species‐responsive silk sericin microcapsules used for antioxidative stress damage
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2020-10-21 , DOI: 10.1002/jemt.23620
Guangshu Wang ₁ , Zhenyu Wang ₁ , Ning Sun ₁ , Yurong Cai ₁ , Xiaogang Yang ₂

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Silk sericin microcapsules loaded with horseradish peroxidase (HRP) are prepared through protein self‐assembly in a green environment containing enzymes to protect liver cells from alcohol damage. Load content and release dynamics of HRP in sericin microcapsules are investigated. The role of HRP‐loaded microcapsules in hydrogen peroxide (H₂O₂) degradation is demonstrated using electrochemical method. Furthermore, the effect of the HRP‐loaded microcapsules on cells and intracellular reactive oxygen species (ROS) level is evaluated using an alcohol damage model in vitro. Results show that HRP can be loaded effectively in the sericin microcapsules and can be released ROS‐responsively from microcapsules. Cell survival rate increases after suffering from alcohol damage due to the presence of HRP‐loaded microcapsule, and the active oxygen content in cells is maintained at a stable level even when it remained in an environment with high alcohol concentration. We believe that the internalized sericin microcapsules maintain HRP activity intracellularly, allow controlled HRP release within a host cell, and show excellent ability in antioxidative stress injury.

中文翻译：

活性氧响应丝胶微胶囊用于抗氧化应激损伤

载有辣根过氧化物酶 (HRP) 的丝胶微胶囊是在含有酶的绿色环境中通过蛋白质自组装制备的，以保护肝细胞免受酒精损伤。研究了丝胶微胶囊中 HRP 的负载含量和释放动力学。负载 HRP 的微胶囊在过氧化氢 (H ₂ O ₂) 使用电化学方法证明了降解。此外，使用体外酒精损伤模型评估了负载 HRP 的微胶囊对细胞和细胞内活性氧 (ROS) 水平的影响。结果表明，HRP 可以有效地加载到丝胶微胶囊中，并且可以响应性地从微胶囊中释放 ROS。由于负载HRP的微胶囊的存在，细胞遭受酒精损伤后存活率提高，即使在高酒精浓度的环境中，细胞内活性氧含量也保持在稳定水平。我们相信内化的丝胶微胶囊在细胞内维持 HRP 活性，允许在宿主细胞内控制 HRP 释放，并显示出优异的抗氧化应激损伤能力。

更新日期：2020-10-21

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