The steroid-induced microRNA let-7 regulates developmental growth by targeting cdc7 in the Drosophila fat body,Insect Science

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The steroid-induced microRNA let-7 regulates developmental growth by targeting cdc7 in the Drosophila fat body
Insect Science ( IF 2.9 ) Pub Date : 2020-10-22 , DOI: 10.1111/1744-7917.12878
Dan-Yan Huang ₁ , Xiao-Ling Xia ₁ , Run Huang ₁ , Sheng Li ₁ , Dong-Wei Yuan ₁ , Su-Ning Liu ₁

Affiliation

In insects, 20-hydroxyecdysone (20E) limits systemic growth by triggering developmental transitions. Previous studies have shown that 20E-induced let-7 exhibits crosstalk with the cell cycle. Here, we examined the underlying molecular mechanisms and physiological functions of 20E-induced let-7 in the fat body, an organ for energy storage and nutrient mobilization which plays a critical role in the larval growth. First, the overexpression of let-7 decreased the body size and led to the reduction of both nucleolus and cell sizes in the larval fat body. In contrast, the overexpression of let-7-Sponge increased the nucleolus and cell sizes. Moreover, we found that cdc7, encoding a conserved protein kinase that controls the endocycle, is a target of let-7. Notably, the mutation of cdc7 in the fat body resulted in growth defects. Overall, our findings revealed a novel role of let-7 in the control of endoreduplication-related growth during larval-prepupal transition in Drosophila.

中文翻译：

类固醇诱导的 microRNA let-7 通过靶向果蝇脂肪体中的 cdc7 来调节发育生长

在昆虫中，20-羟基蜕皮激素 (20E) 通过触发发育转变来限制全身生长。先前的研究表明，20E 诱导的 let-7 会与细胞周期发生串扰。在这里，我们研究了 20E 诱导的 let-7 在脂肪体中的潜在分子机制和生理功能，脂肪体是一种在幼虫生长中起关键作用的能量储存和营养动员器官。首先，let-7 的过表达减小了体型并导致幼虫脂肪体中的核仁和细胞大小都减小。相反，let-7-Sponge 的过表达增加了核仁和细胞大小。此外，我们发现编码控制内环的保守蛋白激酶的cdc7是 let-7 的靶标。值得注意的是，cdc7的突变在脂肪体中导致生长缺陷。总体而言，我们的研究结果揭示了 let-7 在果蝇幼虫 - 预蛹过渡期间控制核内复制相关生长的新作用。

更新日期：2020-10-22

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