Repressor‐Like On‐Off Regulation of Protein Expression by the DNA‐Binding Transcription Activator‐Like Effector in T7 Promoter‐Based Cell‐Free Protein Synthesis,ChemBioChem

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Repressor‐Like On‐Off Regulation of Protein Expression by the DNA‐Binding Transcription Activator‐Like Effector in T7 Promoter‐Based Cell‐Free Protein Synthesis
ChemBioChem ( IF 2.6 ) Pub Date : 2020-10-21 , DOI: 10.1002/cbic.202000591
Masafumi Sakono ₁ , Ryoto Hayakawa ₁

Affiliation

Control of the T7 promoter system: TALE selectively suppresses protein expression through target‐site‐specific DNA recognition and DNA binding. As competitive inhibition of TALE binding restored protein synthesis levels, our results show that TALE can regulate the expression of the T7 promoter system by DNA recognition.

中文翻译：

基于 T7 启动子的无细胞蛋白质合成中 DNA 结合转录激活剂样效应子对蛋白质表达的阻遏样开关调节

T7 启动子系统的控制：TALE 通过目标位点特异性 DNA 识别和 DNA 结合选择性地抑制蛋白质表达。由于 TALE 结合的竞争性抑制恢复了蛋白质合成水平，我们的结果表明 TALE 可以通过 DNA 识别调节 T7 启动子系统的表达。

更新日期：2020-10-21

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