A rheological approach to identify efficient biopolymer producing bacteria,Biotechnology and Bioengineering

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A rheological approach to identify efficient biopolymer producing bacteria
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2020-10-22 , DOI: 10.1002/bit.27595
Sara Malvar ₁ , Letícia O B Cardoso _{2,

3} , Bruno Karolski ₃ , Elen A Perpetuo _{3,

4} , Bruno S Carmo ₁ , Julio R Meneghini ₁

Affiliation

This study investigates the relationship between collective motion and propulsion of bacterial consortia and their biopolymer production efficiency. Rheological tests were conducted for suspensions of two different methanotrophic bacterial consortia obtained after enrichment of sediment samples from mangrove sites in Brazil. We considered the linear viscoelasticity region and analyzed the values of storage and loss moduli as functions of days of cultivation, for different values of the volume fraction. The suspensions' rheological behaviors reflected the bacterial growth stage. We found that the formation of structures over time in some types of consortia can hinder the movement of bacteria in the search for nutrients. The change in complex viscosity of the two consortia followed a different and rich behavior that appears to be closely related to their capacity to capture methane. Our analysis showed a possible correlation between collective motion, viscosity reduction, and biopolymer production. The pieces of evidence from this study suggest that the efficiency of bacterial motion is directly related to biopolymer production, and this could facilitate the process of identifying the best consortium of biopolymer producing bacteria.

中文翻译：

一种识别高效生物聚合物生产细菌的流变学方法

本研究调查了细菌聚生体的集体运动和推进与其生物聚合物生产效率之间的关系。对巴西红树林沉积物样品富集后获得的两种不同甲烷氧化菌群的悬浮液进行了流变测试。我们考虑了线性粘弹性区域，并针对不同的体积分数值分析了作为培养天数的函数的存储和损失模量的值。悬浮液的流变行为反映了细菌的生长阶段。我们发现，随着时间的推移，某些类型的聚生体中结构的形成会阻碍细菌寻找营养的运动。两个财团的复数粘度变化遵循不同且丰富的行为，这似乎与其捕获甲烷的能力密切相关。我们的分析表明集体运动、粘度降低和生物聚合物生产之间可能存在相关性。这项研究的证据表明，细菌运动的效率与生物聚合物的生产直接相关，这可以促进确定最佳生物聚合物生产细菌的过程。

更新日期：2020-10-22

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