Studies on neutrophil‐based nanotherapeutic engineering have shown great potentials in treating infection and inflammation disorders. Conventional neutrophil labeling methods are time‐consuming and often result in undesired contamination and activation since neutrophils are terminal‐differentiated cells with a half‐life span of only 7 h. A simple, fast, and biocompatible strategy to construct engineered neutrophils is highly desirable but remains difficult to achieve. In this study, we present an AIEgen‐lipid conjugate, which can efficiently label harvested neutrophils in 30 s with no washing step required. This fast labeling method does not affect the activation and transmigration property of neutrophils, which has been successfully used to monitor neutrophil behaviors such as the chemotaxis process and migrating function towards inflammation sites both in vitro and in vivo, offering a tantalizing prospect for neutrophil‐based nanotherapeutics studies.

中文翻译：

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AIEgen-脂质偶联物可快速标记中性粒细胞并监测其行为

基于中性粒细胞的纳米治疗工程的研究显示出在治疗感染和炎症疾病方面的巨大潜力。常规的嗜中性白细胞标记方法很费时，并且经常导致不希望的污染和激活，因为嗜中性白细胞是终末分化的细胞，半衰期仅为7 h。构造工程中性粒细胞的简单，快速和生物相容性策略是非常可取的，但仍然难以实现。在这项研究中，我们提出了一种AIEgen-脂质缀合物，它可以在30 s内有效标记收获的中性粒细胞，而无需洗涤步骤。这种快速标记方法不会影响嗜中性粒细胞的活化和转运特性，