当前位置: X-MOL 学术Anal. Chem. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Toward Rapid Screening of Liver Grafts at the Operating Room Using Mid-infrared Spectroscopy
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-10-21 , DOI: 10.1021/acs.analchem.0c02735
David Pérez-Guaita 1 , Marta Moreno-Torres 2 , Ramiro Jover 2, 3, 4 , Eugenia Pareja 2, 5 , Bernhard Lendl 6 , Julia Kuligowski 7 , Guillermo Quintás 8, 9 , Jose Vicente Castell 2, 3, 4
Affiliation  

The estimation of steatosis in a liver graft is mandatory prior to liver transplantation, as the risk of graft failure increases with the level of infiltrated fat. However, the assessment of liver steatosis before transplantation is typically based on a qualitative or semiquantitative characterization by visual inspection and palpation and histological analysis. Thus, there is an unmet need for transplantation surgeons to have access to a diagnostic tool enabling an in situ fast classification of grafts prior to extraction. In this study, we have assessed an attenuated total reflection–Fourier transform infrared (ATR–FTIR) spectroscopic method compatible with the requirements of an operation room for the evaluation of the lipid contents in human livers. A set of 20 human liver biopsies obtained from organs intended for transplantation were analyzed by expert pathologists, ATR–FTIR spectroscopy, lipid biochemical analysis, and UPLC–ESI(+/−)TOFMS for lipidomic profiling. Comparative analysis of multisource data showed strong correlations between ATR–FTIR, clinical, and lipidomic information. Results show that ATR–FTIR captures a global picture of the lipid composition of the liver, along with information for the quantification of the triradylglycerol content in liver biopsies. Although the methodology performance needs to be further validated, results support the applicability of ATR–FTIR for the in situ determination of the grade of liver steatosis at the operation room as a fast, quantitative method, as an alternative to the qualitative and subjective pathological examination.

中文翻译:

使用中红外光谱技术在手术室中快速筛查肝移植物

在肝移植之前,必须对肝移植物中的脂肪变性进行估计,因为移植物失败的风险会随着脂肪含量的增加而增加。然而,移植前肝脂肪变性的评估通常基于通过目测和触诊以及组织学分析的定性或半定量表征。因此,迫切需要移植外科医生使用诊断工具,以便能够在提取前对移植物进行原位快速分类。在这项研究中,我们评估了一种衰减的全反射傅里叶变换红外(ATR-FTIR)光谱法,该方法与手术室的要求相一致,可用于评估人体肝脏中的脂质含量。通过专家病理学家,ATR-FTIR光谱,脂质生化分析和UPLC-ESI(+/-)TOFMS分析了用于器官移植的20份人类肝脏活检组织,以进行脂质组分析。对多源数据的比较分析显示,ATR-FTIR,临床和脂质组学信息之间具有很强的相关性。结果表明,ATR–FTIR可以捕获肝脏脂质成分的整体图像,以及肝脏活检组织中三甲酰甘油含量的定量信息。尽管该方法的性能需要进一步验证,但结果支持ATR–FTIR作为一种快速,定量的方法,可在手术室中现场确定肝脏脂肪变性的级别,作为定性和主观病理学检查的替代方法。
更新日期:2020-11-03
down
wechat
bug