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Dual Colorimetric and Ratiometric Fluorescent Responses for the Determination of Glutathione Based on Fluorescence Quenching and Oxidase-Like Activity of MnO2 Nanosheets
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-10-20 , DOI: 10.1021/acssuschemeng.0c04345 Hui Li 1 , Ziyi Wang 1 , Jingye Zhao 1 , Yuwei Guan 1 , Yueying Liu 1
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-10-20 , DOI: 10.1021/acssuschemeng.0c04345 Hui Li 1 , Ziyi Wang 1 , Jingye Zhao 1 , Yuwei Guan 1 , Yueying Liu 1
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A dual colorimetric and ratiometric fluorescent assay is constructed for the detection of glutathione (GSH) based on MnO2 nanosheets (MnO2 NS). Amplex Red (AR) and curcumin (CUR) fluorescence-pairing probes have opposite responses to MnO2 NS with fluorescence quenching and oxidase-like property. MnO2 nanosheets can largely quench the fluorescence of curcumin. However, the fluorescence of oxidized AR (oxAR) was obviously raised via the catalytic oxidation reaction of no fluorescent AR. The color of the solution gradually changes from yellow to orange. However, MnO2 nanosheets lose the fluorescence quenching capacity and oxidase-like property after the addition of GSH. Therefore, the fluorescence intensity of curcumin shows an obvious increase and the intensity of oxAR a decrease, accompanied by the restoration of the solution color from orange to yellow. For MnO2 nanosheets, the linear relationship between the absorbance ratio of the two substrates and the GSH concentration (50–15 000 nM) is obtained with the detection limit of 16 nM. Besides, the ratiometric fluorescent method has a good linear range from 10 to 5000 nM for GSH. The detection limit is as low as 4.2 nM. Further, this method can not only be used for the determination of GSH in an aqueous solution but also shows a high responsive ability to GSH in human serum. This result indicates its potential in bioanalysis and disease diagnosis.
中文翻译：
MnO 2纳米片的荧光猝灭和类似氧化酶的活性用于比色法和比色法测定谷胱甘肽的荧光响应
基于MnO 2纳米片（MnO 2 NS），构建了比色和比例荧光双重测定法来检测谷胱甘肽（GSH ）。Amplex Red（AR）和姜黄素（CUR）荧光配对探针对MnO 2 NS具有相反的响应，具有荧光猝灭和类似氧化酶的特性。MnO 2纳米片可以大大抑制姜黄素的荧光。然而，通过无荧光AR的催化氧化反应，明显提高了氧化AR（oxAR）的荧光。溶液的颜色从黄色逐渐变为橙色。但是，MnO 2加入GSH后，纳米片失去了荧光猝灭能力和类似氧化酶的性质。因此，姜黄素的荧光强度显示出明显的增加，而oxAR的强度降低，伴随着溶液颜色从橙色恢复为黄色。对于MnO 2纳米片，两种底物的吸光度比与GSH浓度（50-15 000 nM）之间的线性关系以16 nM的检出限获得。此外，对于GSH，比例荧光法具有10到5000 nM的良好线性范围。检测限低至4.2 nM。此外，该方法不仅可用于测定水溶液中的谷胱甘肽，而且对人血清中的谷胱甘肽具有高响应能力。该结果表明其在生物分析和疾病诊断中的潜力。
更新日期：2020-11-02
中文翻译：
MnO 2纳米片的荧光猝灭和类似氧化酶的活性用于比色法和比色法测定谷胱甘肽的荧光响应
基于MnO 2纳米片（MnO 2 NS），构建了比色和比例荧光双重测定法来检测谷胱甘肽（GSH ）。Amplex Red（AR）和姜黄素（CUR）荧光配对探针对MnO 2 NS具有相反的响应，具有荧光猝灭和类似氧化酶的特性。MnO 2纳米片可以大大抑制姜黄素的荧光。然而，通过无荧光AR的催化氧化反应，明显提高了氧化AR（oxAR）的荧光。溶液的颜色从黄色逐渐变为橙色。但是，MnO 2加入GSH后，纳米片失去了荧光猝灭能力和类似氧化酶的性质。因此，姜黄素的荧光强度显示出明显的增加，而oxAR的强度降低，伴随着溶液颜色从橙色恢复为黄色。对于MnO 2纳米片，两种底物的吸光度比与GSH浓度（50-15 000 nM）之间的线性关系以16 nM的检出限获得。此外，对于GSH，比例荧光法具有10到5000 nM的良好线性范围。检测限低至4.2 nM。此外，该方法不仅可用于测定水溶液中的谷胱甘肽，而且对人血清中的谷胱甘肽具有高响应能力。该结果表明其在生物分析和疾病诊断中的潜力。
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