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Self-Driven “Microfiltration” Enabled by Porous Superabsorbent Polymer (PSAP) Beads for Biofluid Specimen Processing and Storage
ACS Materials Letters ( IF 9.6 ) Pub Date : 2020-10-21 , DOI: 10.1021/acsmaterialslett.0c00348
Wensi Chen 1 , Ting Wang 1 , Zeou Dou 1 , Xing Xie 1
Affiliation  

A remote collection of biofluid specimens such as blood and urine remains a great challenge due to the requirement of continuous refrigeration. Without proper temperature regulation, the rapid degradation of analytical targets in the specimen may compromise the accuracy and reliability of the testing results. In this study, we develop porous superabsorbent polymer (PSAP) beads for fast and self-driven “microfiltration” of biofluid samples. This treatment effectively separates small analytical targets (e.g., glucose, catalase, and bacteriophage) and large undesired components (e.g., bacteria and blood cells) in the biofluids by capturing the former inside and excluding the latter outside the PSAP beads. We have successfully demonstrated that this treatment can reduce sample volume, self-aliquot the liquid sample, avoid microbial contamination, separate plasma from blood cells, stabilize target species inside the beads, and enable long-term storage at room temperature. Potential practical applications of this technology can provide an alternative sample collection and storage approach for medically underserved areas.

中文翻译:

多孔超吸收性聚合物(PSAP)磁珠实现生物液体样品加工和存储的自驱动“微滤”

由于需要连续冷藏,因此远程采集生物流体标本(例如血液和尿液)仍然是一个巨大的挑战。如果没有适当的温度调节,样品中分析目标的快速降解可能会损害测试结果的准确性和可靠性。在这项研究中,我们开发了多孔超吸收性聚合物(PSAP)珠粒,用于生物流体样品的快速和自驱动“微滤”。这种处理有效地分离了小的分析目标(例如,葡萄糖,过氧化氢酶和噬菌体)和大的不需要的成分(例如,捕获生物流体中的细菌和血细胞),方法是捕获前者的内部,而将后者排除在PSAP珠之外。我们已经成功地证明了这种处理方法可以减少样品量,自动等分液体样品,避免微生物污染,将血浆与血细胞分离,稳定微珠内的目标物种以及在室温下长期保存。该技术的潜在实际应用可以为医疗欠佳的地区提供一种替代的样本收集和存储方法。
更新日期:2020-11-02
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