SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution,ACS Central Science

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SARS-CoV-2 Proteome Microarray for Mapping COVID-19 Antibody Interactions at Amino Acid Resolution
ACS Central Science ( IF 12.7 ) Pub Date : 2020-10-21 , DOI: 10.1021/acscentsci.0c00742
Hongye Wang ₁ , Xian Wu ₂ , Xiaomei Zhang ₁ , Xin Hou ₂ , Te Liang ₁ , Dan Wang ₁ , Fei Teng ₃ , Jiayu Dai ₁ , Hu Duan ₁ , Shubin Guo ₃ , Yongzhe Li ₂ , Xiaobo Yu ₁

Affiliation

Comprehensive profiling of humoral antibody response to severe acute respiratory syndrome (SARS) coronavirus-2 (CoV-2) proteins is essential in understanding the host immunity and in developing diagnostic tests and vaccines. To address this concern, we developed a SARS-CoV-2 proteome peptide microarray to analyze antibody interactions at the amino acid resolution. With the array, we demonstrate the feasibility of employing SARS-CoV-1 antibodies to detect the SARS-CoV-2 nucleocapsid phosphoprotein. The first landscape of B-cell epitopes for SARS-CoV-2 IgM and IgG antibodies in the serum of 10 coronavirus disease of 2019 (COVID-19) patients with early infection is also constructed. With array data and structural analysis, a peptide epitope for neutralizing antibodies within the SARS-CoV-2 spike receptor-binding domain’s interaction interface with the angiotensin-converting enzyme 2 receptor was predicted. All the results demonstrate the utility of our microarray as a platform to determine the changes of antibody responses in COVID-19 patients and animal models as well as to identify potential targets for diagnosis and treatment.

中文翻译：

SARS-CoV-2蛋白质组微阵列，用于以氨基酸分辨率映射COVID-19抗体相互作用。

对严重急性呼吸综合征（SARS）冠状病毒2（CoV-2）蛋白的体液抗体反应的全面分析对于理解宿主免疫力以及开发诊断测试和疫苗至关重要。为了解决这一问题，我们开发了SARS-CoV-2蛋白质组肽微阵列，以氨基酸分辨率分析抗体相互作用。使用该阵列，我们证明了使用SARS-CoV-1抗体检测SARS-CoV-2核衣壳磷蛋白的可行性。还构建了2019年感染早期10例冠状病毒病（COVID-19）的患者血清中SARS-CoV-2 IgM和IgG抗体的B细胞表位的第一个图谱。利用阵列数据和结构分析，预测了用于中和SARS-CoV-2突触受体结合域与血管紧张素转化酶2受体相互作用界面内抗体的肽表位。所有结果都证明了我们的微阵列可作为确定COVID-19患者和动物模型中抗体反应变化以及确定诊断和治疗潜在靶标的平台。

更新日期：2020-12-23

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