We determined the complete sequence of the mitochondrial DNA (mtDNA) of a parasite discovered between the subcutaneous tissue and the peritoneum of an African nocturnal non-human primate (NHP). The parasite and host sequences were obtained by a combination of Sanger sequencing and nanopore MinION techniques. Analyses of mtDNA gene arrangements and sequences unambiguously showed that the parasite investigated was the pentastomid Armillifer armillatus, also commonly named the tongue worm. The full-length mitochondrial genome of A. armillatus, measuring 16,706 bp in length, contains 13 protein-coding genes, 2 ribosomal RNA genes, and 22 transfer RNA genes, an arrangement identical to that of previously described pentastomid mitochondrial genomes. We describe here the second full mitochondrial genome of A. armillatus to date. To identify the NHP host, maximum likelihood phylogenetic analyses of a 441-bp fragment on the 12S rDNA gene and of a 1,140-bp fragment of the mitochondrial cytochrome b strongly support clustering with the African lorisid Perodicticus potto, a species that has rarely been reported as an intermediate host of this parasite.

我们确定了非洲夜间非人类灵长类动物（NHP）的皮下组织和腹膜之间发现的寄生虫的线粒体DNA（mtDNA）的完整序列。寄生虫和宿主序列是通过Sanger测序和纳米孔MinION技术的组合获得的。mtDNA基因排列和序列的明确分析表明，所研究的寄生虫是五足虫Armillifer armillatus，也通常称为舌虫。犰狳的全长线粒体基因组长度为16,706 bp的DNA包含13个蛋白质编码基因，2个核糖体RNA基因和22个转移RNA基因，其排列与先前描述的五粒线粒体线粒体基因组相同。我们在这里描述了迄今第二个完整的棉铃虫线粒体基因组。为了鉴定NHP宿主，对12S rDNA基因上的441 bp片段和线粒体细胞色素b的1,140 bp片段的最大似然系统发育分析强烈支持与非洲鸢尾Perodicticus potto成簇，该物种鲜有报道作为这种寄生虫的中间宿主。