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Even lobar deposition of poorly soluble gold nanoparticles (AuNPs) is similar to that of soluble silver nanoparticles (AgNPs)
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2020-10-20 , DOI: 10.1186/s12989-020-00384-w Hoi Pin Kim 1 , Jin Kwon Kim 2 , Mi Seong Jo 1 , Jung Duck Park 3 , Kangho Ahn 2 , Mary Gulumian 4, 5, 6 , Günter Oberdörster 7 , Il Je Yu 1, 8
Particle and Fibre Toxicology ( IF 10 ) Pub Date : 2020-10-20 , DOI: 10.1186/s12989-020-00384-w Hoi Pin Kim 1 , Jin Kwon Kim 2 , Mi Seong Jo 1 , Jung Duck Park 3 , Kangho Ahn 2 , Mary Gulumian 4, 5, 6 , Günter Oberdörster 7 , Il Je Yu 1, 8
Affiliation
Information on particle deposition, retention, and clearance is important when evaluating the risk of inhaled nanomaterials to human health. The revised Organization Economic Cooperation and Development (OECD) inhalation toxicity test guidelines now require lung burden measurements of nanomaterials after rodent subacute and sub-chronic inhalation exposure (OECD 412, OECD 413) to inform on lung clearance behavior and translocation after exposure and during post-exposure observation (PEO). Lung burden measurements are particularly relevant when the testing chemical is a solid poorly soluble nanomaterial. Previously, the current authors showed that total retained lung burden of inhaled soluble silver nanoparticles (AgNPs) could be effectively measured using any individual lung lobe. Accordingly, the current study investigated the evenness of deposition/retention of poorly soluble gold nanoparticles (AuNPs) after 1 and 5 days of inhalation exposure. Rats were exposed nose-only for 1 or 5 days (6 h/day) to an aerosol of 11 nm well-dispersed AuNPs. Thereafter, the five lung lobes were separated and the gold concentrations measured using an inductively coupled plasma-mass spectrophotometer (ICP-MS). The results showed no statistically significant difference in the AuNP deposition/retention among the different lung lobes in terms of the gold mass per gram of lung tissue. Thus, it would seem that any rat lung lobe can be used for the lung burden analysis after short or long-term NP inhalation, while the other lobes can be used for collecting and analyzing the bronchoalveolar lavage fluid (BALF) and for the histopathological analysis. Therefore, combining the lung burden measurement, histopathological tissue preparation, and BALF assay from one rat can minimize the number of animals used and maximize the number of endpoints measured.
中文翻译:
甚至难溶性金纳米颗粒(AuNPs)的大叶沉积也类似于可溶性银纳米颗粒(AgNPs)的大叶沉积
在评估吸入的纳米材料对人体健康的风险时,有关颗粒沉积,保留和清除的信息很重要。修订后的《组织经济合作与发展组织(OECD)吸入毒性测试指南》现在要求在啮齿动物亚急性和亚慢性吸入暴露后测量纳米材料的肺负荷(OECD 412,OECD 413),以告知暴露后和暴露后的肺清除行为和移位。曝光观察(PEO)。当测试化学品是固体难溶性纳米材料时,肺负荷测量尤其重要。以前,当前作者表明,使用任何单个肺叶都可以有效地测量吸入可溶性银纳米颗粒(AgNP)的总保留肺负荷。因此,当前的研究调查了吸入暴露1天和5天后难溶性金纳米颗粒(AuNP)的沉积/保留均匀性。大鼠仅用鼻子暴露于11 nm良好分散的AuNPs气雾剂中1或5天(6小时/天)。此后,分离五个肺叶,并使用感应耦合等离子体质谱分光光度计(ICP-MS)测量金浓度。结果显示,就每克肺组织的金质量而言,不同肺叶之间的AuNP沉积/保留率无统计学意义上的显着差异。因此,短期或长期吸入NP后,任何大鼠肺叶似乎均可用于肺负荷分析,而其他肺叶可用于收集和分析支气管肺泡灌洗液(BALF)以及用于组织病理学分析。因此,
更新日期:2020-10-20
中文翻译:
甚至难溶性金纳米颗粒(AuNPs)的大叶沉积也类似于可溶性银纳米颗粒(AgNPs)的大叶沉积
在评估吸入的纳米材料对人体健康的风险时,有关颗粒沉积,保留和清除的信息很重要。修订后的《组织经济合作与发展组织(OECD)吸入毒性测试指南》现在要求在啮齿动物亚急性和亚慢性吸入暴露后测量纳米材料的肺负荷(OECD 412,OECD 413),以告知暴露后和暴露后的肺清除行为和移位。曝光观察(PEO)。当测试化学品是固体难溶性纳米材料时,肺负荷测量尤其重要。以前,当前作者表明,使用任何单个肺叶都可以有效地测量吸入可溶性银纳米颗粒(AgNP)的总保留肺负荷。因此,当前的研究调查了吸入暴露1天和5天后难溶性金纳米颗粒(AuNP)的沉积/保留均匀性。大鼠仅用鼻子暴露于11 nm良好分散的AuNPs气雾剂中1或5天(6小时/天)。此后,分离五个肺叶,并使用感应耦合等离子体质谱分光光度计(ICP-MS)测量金浓度。结果显示,就每克肺组织的金质量而言,不同肺叶之间的AuNP沉积/保留率无统计学意义上的显着差异。因此,短期或长期吸入NP后,任何大鼠肺叶似乎均可用于肺负荷分析,而其他肺叶可用于收集和分析支气管肺泡灌洗液(BALF)以及用于组织病理学分析。因此,