The synthesis of metal oxide nanoparticles with the use of medicinal plant extract is a promising alternative to the conventional chemical method. This work aimed to synthesize zinc oxide nanoparticles using a green approach from indigenous “Koseret” Lippia adoensis leaf extract which is an endemic medicinal plant and cultivated in home gardens of different regions of Ethiopia. The biosynthesized zinc oxide nanoparticles were characterized using thermogravimetric analysis, X-ray diffraction, scanning electron microscopy-energy dispersive spectroscopy, transmission electron microscopy, ultraviolet-visible spectroscopy, and Fourier transform infrared spectroscopy. Furthermore, this study also evaluated the antibacterial activity of the synthesized ZnO nanoparticles against clinical and standard strains of Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and Enterococcus faecalis by the disc diffusion method. According to the result of this study, ZnO nanoparticles synthesized using Lippia adoensis leaf extract showed promising result against both Gram-positive and Gram-negative bacterial strains with a maximum inhibition zone of 14 mm and 12 mm, respectively, using uncalcinated form of the synthesized ZnO nanoparticles.

中文翻译：

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用 Lippia adoensis (Koseret) 叶提取物合成氧化锌纳米颗粒及其抗菌活性评价

使用药用植物提取物合成金属氧化物纳米颗粒是传统化学方法的一种有前景的替代方法。这项工作旨在使用绿色方法从本土的“Koseret”Lippia adoensis 叶提取物合成氧化锌纳米粒子，这是一种地方性药用植物，在埃塞俄比亚不同地区的家庭花园中种植。使用热重分析、X 射线衍射、扫描电子显微镜-能量色散光谱、透射电子显微镜、紫外-可见光谱和傅里叶变换红外光谱对生物合成的氧化锌纳米粒子进行了表征。此外，本研究还评估了合成的 ZnO 纳米颗粒对大肠杆菌、肺炎克雷伯菌、肺炎克雷伯菌的临床和标准菌株的抗菌活性。通过圆盘扩散法检测金黄色葡萄球菌和粪肠球菌。根据这项研究的结果，使用未煅烧形式的合成氧化锌纳米颗粒合成的氧化锌纳米颗粒对革兰氏阳性和革兰氏阴性菌株均显示出有希望的结果，最大抑菌圈分别为 14 毫米和 12 毫米。氧化锌纳米颗粒。