The current study aimed to investigate the damage of long-term high concentrate diet feeding pattern on Yak jejunal structure, physiological function and protein composition during cold season. Twelve Datong male Yak (Bos grunniens) with the same age from cold season were randomly selected and slaughtered to determine Yak jejunal digestive enzyme activity, morphology and protein composition on different feeding patterns in Tibetan Plateau. The results showed that Yak jejunum digestive enzyme activity and morphology of grazing reared group were better than those in the intensively reared group. A total of 96 differentially expressed proteins were identified by label-free Mass Spectrometry (MS), which could be concluded to two predominant themes: protein structure and inflammatory response. Nine differentially expressed proteins were correlated in Yak jejunum damage in different feeding patterns. According to our study, feeding patterns resulted in the differences in Yak jejunum physiological function, morphology and protein composition. Our results confirmed that feeding of long-term high concentrate diet could damage the jejunum epithelial morphology and function.

中文翻译：

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高比例浓缩饲料对寒冷季节Ya牛空肠结构，生理功能和蛋白质组成的影响

当前的研究旨在调查长期高浓度饮食喂养方式对寒冷季节Ya牛空肠结构，生理功能和蛋白质组成的损害。随机选择十二只同龄冷季大同雄性Ya牛（Bos grunniens）并宰杀，以测定青藏高原不同饲养方式的Ya牛空肠消化酶活性，形态和蛋白质组成。结果表明，放牧rear牛的Ya牛空肠消化酶活性和形态均好于精养group牛。通过无标记质谱（MS）鉴定出总共96种差异表达的蛋白质，这可以归结为两个主要主题：蛋白质结构和炎症反应。九种差异表达的蛋白质在不同喂养方式下对Ya牛空肠的损害相关。根据我们的研究，喂养方式导致空肠k牛生理功能，形态和蛋白质组成的差异。我们的结果证实长期高浓度饮食喂养可能会损害空肠上皮的形态和功能。