SummaryCulture conditions have a profound effect on the quality of in vitro-produced embryos. Co-culturing embryos with somatic cells has some beneficial effects on embryonic development. Considering the ability of stem cells to secrete a broad range of growth factors with different biological activities, we hypothesized that bovine amniotic membrane stem cells (bAMSCs) might be superior to bovine oviduct epithelial cells (BOECs) in supporting embryonic development and enhancing their cryo-survival. Bovine abattoir-derived oocytes were matured and fertilized in vitro. The resultant presumptive zygotes were then cultured up to the blastocyst stage in the following groups: (i) co-culture with bAMSCs, (ii) co-culture with BOECs, and (iii) cell-free culture (Con). Embryos that reached the blastocyst stage were vitrified and warmed, and their post-warming re-expansion, survival and hatching rates were evaluated after 72 h culture. Results showed that the cleavage, blastocyst, and 2 h post-warming re-expansion rates of embryos did not differ between groups. However, their survival rates in BOEC and bAMSC groups were significantly higher compared with the control (72.7, 75.6 and 37.5%, respectively, P < 0.05). In conclusion, our results showed that the cryo-survivability of IVF-derived bovine embryos could be improved through co-culturing with bAMSCs. Moreover, considering the possibility to provide multiple passages from bAMSCs compared with BOECs, due to their stemness properties and their ability to produce growth factors, the use of bAMSCs is a good alternative to BOECs in embryo co-culture systems.

中文翻译：

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胚胎与牛羊膜干细胞共培养可以提高体外受精牛胚泡的冷冻存活率，与与牛输卵管上皮细胞共培养相当

总结文化条件对品质的影响深远体外- 产生的胚胎。与体细胞共培养胚胎对胚胎发育有一些有益的影响。考虑到干细胞能够分泌多种具有不同生物活性的生长因子，我们假设牛羊膜干细胞 (bAMSCs) 在支持胚胎发育和增强其冷冻能力方面可能优于牛输卵管上皮细胞 (BOECs)。生存。牛屠宰场来源的卵母细胞成熟并受精体外. 然后将所得推定受精卵培养至以下组的囊胚阶段：（i）与 bAMSCs 共培养，（ii）与 BOEC 共培养，和（iii）无细胞培养（Con）。对达到囊胚期的胚胎进行玻璃化加温，培养72 h后评价其加温后的复膨率、存活率和孵化率。结果表明，各组间胚胎的卵裂率、囊胚率和升温后2 h的再膨胀率没有差异。然而，它们在 BOEC 和 bAMSC 组的存活率显着高于对照组（分别为 72.7、75.6 和 37.5%，磷< 0.05)。总之，我们的研究结果表明，IVF 衍生的牛胚胎的低温存活能力可以通过与 bAMSCs 共培养来提高。此外，考虑到与 BOECs 相比，bAMSCs 可以提供多个通道，由于它们的干性特性和产生生长因子的能力，在胚胎共培养系统中使用 bAMSCs 是 BOECs 的一个很好的替代品。