The objective of this study was to assess the effect of a novel corn-expressed phytase (CEP) on growth, nutrients digestibility, bone characteristics and fecal microbiota of pigs fed with very low-protein, -calcium (Ca) and -phosphorous (P) diets. Forty-eight barrows were subjected to 6 groups for 4 weeks: positive control-adequate protein (PC), negative control-reduced protein (NC), NC + low-dose CEP, i.e., 2000 FTU/kg (LD), NC + high-dose CEP, i.e., 4000 FTU/kg (HD), LD with 0.12% unit reduced Ca and 0.15% unit reduced available P (LDR), and HD with 0.12% unit reduced Ca and 0.15% unit reduced available P (HDR). Compared to NC, LD and HDR had a higher average daily gain (ADG) and gain:protein ratio (G:P), HD and HDR had greater apparent fecal digestibility of Ca and P and bone mineral density and LDR and HDR had lower serum osteocalcin. The feces of LD was enriched in Lachnospiraceae, while the HD had a higher abundance of Succinvibrio and LDR had a higher abundance of Bifidobacterium and Actinobacteria. In conclusion, supplementation of protein-restricted diets with a CEP decreased their negative effects on ADG and G:P ratio, increased the digestibility of Ca and P regardless of the levels of these minerals in the diet, improved bone characteristics and produced differential effects on fecal bacterial population.

中文翻译：

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玉米表达的新型植酸酶可改善日粮添加量非常低的猪的日增重，蛋白质效率比和养分消化率，并改变粪便微生物群

这项研究的目的是评估新型玉米表达的肌醇六磷酸酶（CEP）对饲喂极低蛋白，-钙（Ca）和-磷（P）的猪的生长，养分消化率，骨骼特征和粪便微生物群的影响。 ）饮食。四十八只公猪分为6组，共4周：阳性对照-适量蛋白（PC），阴性对照-还原蛋白（NC），NC +低剂量CEP，即2000 FTU / kg（LD），NC +大剂量CEP，即4000 FTU / kg（HD），LD的Ca减少0.12％，有效磷减少0.15％（LDR），HD的Ca减少0.12％，HD减少0.15％（HDR） ）。与NC相比，LD和HDR的平均日增重（ADG）和增重：蛋白质比（G：P）更高，HD和HDR的粪便中Ca和P的表观消化率更高，骨矿物质密度更高，LDR和HDR的血清更低骨钙素。琥珀酸弧菌和LDR的双歧杆菌和放线菌的含量较高。总之，在限制蛋白质的饮食中添加CEP可以降低其对ADG和G：P比率的负面影响，提高Ca和P的消化率，而与饮食中这些矿物质的含量无关，可以改善骨骼特性，并在饮食中产生不同的影响。粪便细菌种群。