Critical Assessment of the Application of Multidetection SEC and AF4 for the Separation of Single-Chain Nanoparticles,ACS Macro Letters

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Critical Assessment of the Application of Multidetection SEC and AF4 for the Separation of Single-Chain Nanoparticles
ACS Macro Letters ( IF 5.1 ) Pub Date : 2020-10-20 , DOI: 10.1021/acsmacrolett.0c00519
Johanna Engelke _{1,

2,

3} , Susanne Boye ₁ , Bryan T Tuten _{3,

4} , Leonie Barner _{3,

4,

5} , Christopher Barner-Kowollik _{3,

4} , Albena Lederer _{1,

2,

6}

Affiliation

The intramolecular chain collapse of linear precursors with systematic variation of molar mass and ligation group density (5, 15, and 30 mol %) into single-chain nanoparticles (SCNPs) was studied by two different separation approaches. The efficiency of size exclusion chromatography with quadruple detection (SEC-D4) was compared to asymmetrical field flow fractionation hyphenated to quintuple detection (AF4-D5) in organic solvent. The application of the unique combination of advanced detection to different separation principles opens up the opportunity to critically evaluate the determination of molar masses and different types of radii for an in-depth understanding of the structural properties affected by the internal folding process. This is achieved by a detailed comparison of assets, drawbacks, and limitations of these approaches based on the systematical screening of different chain lengths and sizes of the precursors and the SCNPs. Furthermore, an alternative strategy for quantitative determination of intramolecular ligation density by a combination of AF4 and UV detection is introduced.

中文翻译：

多检测 SEC 和 AF4 在单链纳米颗粒分离中应用的批判性评估

通过两种不同的分离方法研究了具有系统变化的摩尔质量和连接基团密度（5、15 和 30 mol%）的线性前体分子内链崩解成单链纳米粒子 (SCNPs)。将具有四重检测的尺寸排阻色谱 (SEC-D4) 的效率与有机溶剂中的非对称场流分级连接到五重检测 (AF4-D5) 的效率进行了比较。将先进检测的独特组合应用于不同的分离原理，为批判性地评估摩尔质量和不同类型半径的确定提供了机会，以深入了解受内部折叠过程影响的结构特性。这是通过详细比较资产、缺点、基于对前体和 SCNP 的不同链长和大小的系统筛选，这些方法的局限性和局限性。此外，还介绍了一种通过 AF4 和 UV 检测相结合来定量测定分子内连接密度的替代策略。

更新日期：2020-11-17

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