Abstract The ecology of a biofilm is a complex function of different factors, including the presence of microbial metabolites excreted by the inhabitants of the biofilm. This study aimed to assess the effect of patulin, and N-(3-oxododecanoyl)-L-homoserine lactone (3-oxo-C12-HSL) on inter-kingdom biofilm formation between a filamentous fungus and bacteria isolated from drinking water. The filamentous fungus Penicillium expansum and the bacteria Acinetobacter calcoaceticus and Methylobacterium oryzae were used as model species. M. oryzae biofilm formation and development was more susceptible to the presence of the quenching molecules than A. calcoaceticus biofilms. Patulin reduced M. oryzae biofilm growth while 3-oxo-C12-HSL caused an increase after 48 h. The presence of P. expansum had a detrimental effect on M. oryzae cell numbers, while an advantageous effect was observed with A. calcoaceticus. The overall results reveal that quorum sensing and quenching molecules have a significant effect on inter-kingdom biofilm formation, especially on bacterial numbers.

中文翻译：

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群体感应和猝灭分子对膨胀青霉和细菌跨界生物膜形成的影响

摘要 生物膜的生态是不同因素的复杂函数，包括生物膜居民排泄的微生物代谢物的存在。本研究旨在评估展曲霉素和 N-(3-oxododecanoyl)-L-高丝氨酸内酯 (3-oxo-C12-HSL) 对从饮用水中分离的丝状真菌和细菌之间的界间生物膜形成的影响。丝状真菌Penicillium expansum 和细菌Acinetobacter calcoacetus 和Methylobacterium oryzae 被用作模型物种。M. oryzae 生物膜的形成和发展比 A.calcoacetus 生物膜更容易受到淬灭分子的影响。展青霉素减少米曲霉生物膜的生长，而 3-oxo-C12-HSL 在 48 小时后导致增加。P. expansum 的存在对 M. oryzae 细胞数量有不利影响，而 A. calcoacetus 观察到了有利的效果。总体结果表明，群体感应和淬灭分子对跨界生物膜形成有显着影响，尤其是对细菌数量。