Structure of neuroendocrine regulatory peptide-2 in membrane-mimicking environments,Peptide Science

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Structure of neuroendocrine regulatory peptide-2 in membrane-mimicking environments
Peptide Science ( IF 1.5 ) Pub Date : 2020-10-19 , DOI: 10.1002/pep2.24206
One‐Sung Park _{1,

2} , Jeong Kyu Bang ₂ , Kyoung‐Seok Ryu ₂ , Eunha Hwang ₂ , Kwan Soo Hong ₂ , Youngjoo Byun ₁ , Chaejoon Cheong ₂ , Young Ho Jeon ₁

Affiliation

Neuroendocrine regulatory peptide-2 (NERP-2) is involved in the maintenance of homeostasis and regulates various metabolic activities. NERP-2 is derived from the VGF neuropeptide precursor, which is induced by various neurotrophins. Despite increasing interest in the functions of NERP-2, the structure of this peptide has not been reported. In this study, the structure of the NERP-2 peptide was investigated using circular dichroism and nuclear magnetic resonance (NMR) spectroscopy. NERP-2 exists as a partial helical structure in buffer solutions, and the helical structure exhibits more stable behaviors in hexafluoroisopropanol (HFIP) or dodecylphosphocholine (DPC) micelle. We determined its 3D structure in both HFIP and DPC micelle solutions using NMR spectroscopy. NERP-2 adopted a predominantly helical structure in HFIP solutions, while only the central portion of the protein was helical in the DPC solutions. The amphipathic nature of helical structure implies the potential structural changes in NERP-2 upon receptor binding.

中文翻译：

膜模拟环境中神经内分泌调节肽2的结构

神经内分泌调节肽2（NERP-2）参与体内平衡的维持并调节各种代谢活动。NERP-2衍生自VGF神经肽前体，它是由多种神经营养蛋白诱导的。尽管人们对NERP-2的功能越来越感兴趣，但尚未报道该肽的结构。在这项研究中，使用圆二色性和核磁共振（NMR）光谱研究了NERP-2肽的结构。NERP-2在缓冲溶液中以部分螺旋结构形式存在，并且该螺旋结构在六氟异丙醇（HFIP）或十二烷基磷酸胆碱（DPC）胶束中表现出更稳定的行为。我们使用NMR光谱法在HFIP和DPC胶束溶液中确定了其3D结构。NERP-2在HFIP解决方案中主要采用了螺旋结构，而在DPC溶液中只有蛋白质的中心部分呈螺旋状。螺旋结构的两亲性质意味着受体结合后NERP-2的潜在结构变化。

更新日期：2020-10-19

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