Host‐induced gene silencing for engineering resistance to Fusarium in soybean,Plant Pathology

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Host‐induced gene silencing for engineering resistance to Fusarium in soybean
Plant Pathology ( IF 2.3 ) Pub Date : 2020-10-19 , DOI: 10.1111/ppa.13299
Cristobal E. B. Pérez _{1,

2} , Glaucia B. Cabral ₁ , Francisco J. L. Aragão _{1,

2}

Affiliation

Soybean is one of the most economically important crops in the world. Its production is affected by several fungal diseases, such as those caused by Fusarium spp., causing significant losses in yield and seed quality. Management interventions are limited, costly, and associated with environmental problems. Host resistance provides a more convenient and cost‐effective approach. Host‐induced gene silencing (HIGS) has been demonstrated to be an alternative strategy to engineer fungus resistance in plants. We have generated transgenic soybean lines with an intron‐hairpin construction in order to express siRNA corresponding to the CYP51B gene from Fusarium oxysporum. Results showed the presence of siRNA corresponding to the F. oxysporum CYP51B gene in both leaves and roots of the transgenic lines. Plants (T₃ generation) were challenged against F. oxysporum and F. graminearum. Disease severity was evaluated and revealed resistance to F. oxysporum with one line, named 3.22, presenting no symptoms. In addition, transgenic lines presented better plant development (height and root growth) when compared to the nontransgenic line. Moreover, transgenic lines revealed better development when inoculated with F. oxysporum.

中文翻译：

宿主诱导的基因沉默对大豆对镰刀菌的工程抗性

大豆是世界上最重要的经济作物之一。它的生产受到几种真菌病的影响，例如镰刀菌引起的那些病，导致产量和种子质量的重大损失。管理干预是有限的，昂贵的并且与环境问题有关。主机抵抗提供了一种更方便且更具成本效益的方法。宿主诱导的基因沉默（HIGS）已被证明是改造植物真菌抗性的另一种策略。我们已经构建了具有内含子-发夹结构的转基因大豆品系，以便表达与来自尖镰孢的CYP51B基因相对应的siRNA 。结果显示对应于F的siRNA的存在。在转基因品系的叶和根中都含有oxysporum CYP51B基因。植物（T ₃代）受到F的攻击。病菌和˚F。菌。评估疾病的严重程度并显示出对F的抵抗力。oxysporum用一条线命名为3.22，无任何症状。此外，与非转基因品系相比，转基因品系表现出更好的植物发育（高度和根系生长）。此外，转基因品系接种F后显示出更好的发育。尖孢菌。

更新日期：2020-10-19

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