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The Sirtuin‐1 relied antioxidant and antiaging activity of 5,5′‐diferulic acid glucoside esters derived from corn bran by enzymatic method
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-10-19 , DOI: 10.1111/jfbc.13519 Jianbin Xue 1 , Xue Sheng 1 , Bing Jie Zhang 2 , Cijia Zhang 1 , Guirong Zhang 1
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2020-10-19 , DOI: 10.1111/jfbc.13519 Jianbin Xue 1 , Xue Sheng 1 , Bing Jie Zhang 2 , Cijia Zhang 1 , Guirong Zhang 1
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Maize is the food crop with the highest total output in the world. However, corn bran is only a by‐product with low price. The 5,5′‐diferulic acid glucoside esters (DFG) were obtained from corn bran using the enzymatic method. DFG showed obvious antioxidant capacity in cell, Caenorhabditis elegans (C. elegans) and in mouse. DFG decreased ROS and MDA content in 500 μM H2O2 stimulated ARPE‐19 cells to 48.6% and 32.2%, respectively. DFG decreased ROS content in C. elegans to 49.1% and MDA content in acute ethanol (50%, 12 ml/kg) stimulated mouse to 30.4%. DFG also increased SOD protein content significantly in cell, C. elegans and mouse to 175.5%, 120.1%, and 126.2%, respectively. DFG significantly extended the lifespan of C. elegans both under heat stress and natural situation. The median survival time was prolonged to 133.3% and 116.7%, respectively. This capacity relied on the SIR‐2.1 activity. SIR‐2.1 is an ortholog of human Sirtuin‐1 (SIRT‐1). DFG also upregulated SIRT‐1 and PCG‐1α expression level obviously in H2O2‐stimulated ARPE‐19 cells (to 134.4% and 127.1%, respectively) and in acute ethanol stimulated mouse eyes (to 135.1% and 111.5%, respectively) and liver (to 123.3% and 113.6%, respectively). These results indicate that DFG has multiple bioactivities. Our research provides a new application prospect of corn bran. And to our best knowledge, this is the first time, the sirtuins‐relied lifespan extension activity of the 5,5′‐diferulic acid extracted from corn bran was reported.
中文翻译：
Sirtuin-1依靠酶法获得了玉米麸皮中5,5'-二硬脂酸葡萄糖苷酯的抗氧化和抗衰老活性
玉米是世界上总产量最高的粮食作物。但是，玉米糠只是价格低廉的副产品。5,5'-二硬脂酸葡萄糖苷酯（DFG）使用酶法从玉米麸获得。DFG在细胞，秀丽隐杆线虫和小鼠中显示出明显的抗氧化能力。DFG将500μMH 2 O 2刺激的ARPE-19细胞中的ROS和MDA含量分别降低至48.6％和32.2％。DFG将线虫中的ROS含量降低至49.1％，将急性乙醇（50％，12 ml / kg）刺激的小鼠中的MDA含量降低至30.4％。DFG还显着增加了秀丽隐杆线虫中SOD的蛋白质含量和鼠标分别为175.5％，120.1％和126.2％。DFG显着延长了秀丽隐杆线虫在热胁迫和自然条件下的寿命。中位生存时间分别延长至133.3％和116.7％。这种能力取决于SIR-2.1活动。SIR-2.1是人类Sirtuin-1（SIRT-1）的直系同源物。DFG在H 2 O 2中也明显上调SIRT-1和PCG-1α表达水平刺激的ARPE-19细胞（分别达到134.4％和127.1％）以及在急性乙醇刺激的小鼠眼中（分别达到135.1％和111.5％）和肝脏（分别达到123.3％和113.6％）。这些结果表明DFG具有多种生物活性。我们的研究为玉米糠的应用提供了新的前景。据我们所知，这是首次报道了从玉米糠中提取的5,5'-二硬脂酸的Sirtuins依赖的寿命延长活性。
更新日期：2020-12-23
中文翻译：
Sirtuin-1依靠酶法获得了玉米麸皮中5,5'-二硬脂酸葡萄糖苷酯的抗氧化和抗衰老活性
玉米是世界上总产量最高的粮食作物。但是，玉米糠只是价格低廉的副产品。5,5'-二硬脂酸葡萄糖苷酯（DFG）使用酶法从玉米麸获得。DFG在细胞，秀丽隐杆线虫和小鼠中显示出明显的抗氧化能力。DFG将500μMH 2 O 2刺激的ARPE-19细胞中的ROS和MDA含量分别降低至48.6％和32.2％。DFG将线虫中的ROS含量降低至49.1％，将急性乙醇（50％，12 ml / kg）刺激的小鼠中的MDA含量降低至30.4％。DFG还显着增加了秀丽隐杆线虫中SOD的蛋白质含量和鼠标分别为175.5％，120.1％和126.2％。DFG显着延长了秀丽隐杆线虫在热胁迫和自然条件下的寿命。中位生存时间分别延长至133.3％和116.7％。这种能力取决于SIR-2.1活动。SIR-2.1是人类Sirtuin-1（SIRT-1）的直系同源物。DFG在H 2 O 2中也明显上调SIRT-1和PCG-1α表达水平刺激的ARPE-19细胞（分别达到134.4％和127.1％）以及在急性乙醇刺激的小鼠眼中（分别达到135.1％和111.5％）和肝脏（分别达到123.3％和113.6％）。这些结果表明DFG具有多种生物活性。我们的研究为玉米糠的应用提供了新的前景。据我们所知，这是首次报道了从玉米糠中提取的5,5'-二硬脂酸的Sirtuins依赖的寿命延长活性。
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