Illumination spot in a flow cytometer is a crucial factor determining the measurement accuracy and stability. The traditional mechanism is to precisely calibrate multiple optical components to convert circular Gaussian beams into elliptical Gaussian beams, making it difficult to shape multiwavelength lasers simultaneously. A diffractive beam shaper for multicolor lasers with high simplicity, only containing one diffractive optical element and one focusing lens is created in this work. It can produce rectangular spots, of which the number, the sizes, and the positions are accurately determined by the incident wavelengths. Demonstrated in the customized microflow cytometer, the coefficient of variations (CV) of the optical signals by the beam shaper are 3.6–6.5%, comparable to those derived from the commercial instrument with 3.3–6.3% CVs. Benefiting from the narrow rectangular spots and the flexibility of diffractively shaped lasers, the measurement of bead sizes with 4–15 μm diameters and the real‐time detection of flow velocity from 0.79 to 9.50 m/s with the CV of <5% are achieved. © 2020 International Society for Advancement of Cytometry

中文翻译：

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用于流式细胞术的多波长激光衍射光束整形器

流式细胞仪中的照明点是决定测量精度和稳定性的关键因素。传统的机制是对多个光学元件进行精确校准，将圆形高斯光束转换为椭圆形高斯光束，因此难以同时对多波长激光器进行整形。在这项工作中创建了一种用于多色激光器的衍射光束整形器，非常简单，仅包含一个衍射光学元件和一个聚焦透镜。它可以产生矩形光斑，光斑的数量、大小和位置由入射波长精确确定。在定制的微流式细胞仪中证明，光束整形器的光学信号的变异系数 (CV) 为 3.6-6.5%，与具有 3.3-6.3% CV 的商业仪器相媲美。受益于窄矩形光斑和衍射形状激光器的灵活性，实现了直径为 4-15 μm 的珠粒尺寸的测量以及流速从 0.79 到 9.50 m/s 的实时检测，CV <5% . © 2020 国际细胞计量学促进会