The unusual dilatation of dermal capillaries and angiogenesis played important roles in psoriasis. Some genes and proteins of dermal mesenchymal stem cells (DMSCs) from psoriasis are abnormal and related to the function of endothelial cells (ECs). The present study was aimed to evaluate whether psoriatic DMSCs could affect adhesion and migration of ECs through neovascularization‐related integrins in psoriasis. Human DMSCs, collected from psoriasis lesions and healthy skin, respectively, were cocultured with human umbilical vein endothelial cells (HUVECs). The expression levels of three integrins, that is, αvβ3, αvβ5, and α5β1 in HUVECs were tested by quantitative real‐time polymerase chain reaction and Western blot analysis. The adhesion and migration of HUVECs were detected by adhesion assay and migration assay. The results showed that in psoriasis group, the expression of αVβ3 and α5β1 of HUVECs markedly increased 2.50‐ and 3.71‐fold in messenger RNA levels, and significantly increased 1.63‐ and 1.92‐fold in protein levels, comparing to healthy control group (all p < .05). But β5 was not significantly different between the two groups (p > .05). In addition, compared with control, psoriatic DMSCs promoted HUVECs adhesion by 1.62‐fold and migration by 2.91‐fold (all p < .05). In conclusion, psoriatic DMSCs impact HUVECs adhesion and migration by upregulating the expression of integrins αVβ3 and α5β1.

中文翻译：

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真皮间充质干细胞通过整合素促进银屑病内皮细胞的粘附和迁移

真皮毛细血管异常扩张和血管生成在银屑病中起重要作用。银屑病真皮间充质干细胞 (DMSCs) 的一些基因和蛋白质异常，与内皮细胞 (ECs) 的功能有关。本研究旨在评估银屑病 DMSCs 是否可以通过银屑病中的新生血管形成相关整合素影响 ECs 的粘附和迁移。分别从银屑病病变和健康皮肤收集的人 DMSC 与人脐静脉内皮细胞 (HUVEC) 共培养。通过定量实时聚合酶链反应和蛋白质印迹分析检测了 HUVEC 中 αvβ3、αvβ5 和 α5β1 三种整联蛋白的表达水平。通过粘附试验和迁移试验检测HUVECs的粘附和迁移。p  < .05）。但β5 在两组之间没有显着差异（p  > .05）。此外，与对照相比，银屑病 DMSCs 促进 HUVECs 粘附 1.62 倍和迁移 2.91 倍（所有p  < .05）。总之，银屑病 DMSCs 通过上调整合素 αVβ3 和 α5β1 的表达来影响 HUVECs 的粘附和迁移。