HClO‐Activated Fluorescence and Photosensitization from an AIE Nanoprobe for Image‐Guided Bacterial Ablation in Phagocytes,Advanced Materials

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HClO‐Activated Fluorescence and Photosensitization from an AIE Nanoprobe for Image‐Guided Bacterial Ablation in Phagocytes
Advanced Materials ( IF 27.4 ) Pub Date : 2020-10-20 , DOI: 10.1002/adma.202005222
Min Wu ₁ , Wenbo Wu ₁ , Yukun Duan ₁ , Xingang Liu ₁ , Meng Wang ₂ , Chi Uyen Phan ₃ , Guobin Qi ₁ , Guping Tang ₃ , Bin Liu _{1,

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Affiliation

Bacteria hiding in host phagocytes are difficult to kill, which can cause phagocyte disorders resulting in local and systemic tissue damage. Effective accumulation of activatable photosensitizers (PSs) in phagocytes to realize selective imaging and on‐demand photodynamic ablation of bacteria is of great scientific and practical interests for precise bacteria diagnosis and treatment. Herein, HClO‐activatable theranostic nanoprobes, DTF‐FFP NPs, for image‐guided bacterial ablation in phagocytes are introduced. DTF‐FFP NPs are prepared by nanoprecipitation of an HClO‐responsive near‐infrared molecule FFP and an efficient PS DTF with aggregation‐induced emission characteristic using an amphiphilic polymer Pluronic F127 as the encapsulation matrix. As an energy acceptor, FFP can quench both fluorescence and production of reactive oxygen species (ROS) of DTF, thus eliminating the phototoxicity of DTF‐FFP NPs in normal cells and tissues. Once delivered to the infection sites, DTF‐FFP NPs light up with red fluorescence and efficiently generate ROS owing to the degradation of FFP by the stimulated release of HClO in phagocytes. The selective activation of fluorescence and photosensitization is successfully confirmed by both in vitro and in vivo results, demonstrating the effectiveness and theranostic potential of DTF‐FFP NPs in precise bacterial therapy.

中文翻译：

来自 AIE 纳米探针的 HClO 激活荧光和光敏化，用于吞噬细胞中的图像引导细菌消融

隐藏在宿主吞噬细胞中的细菌很难被杀死，这会导致吞噬细胞功能紊乱，导致局部和全身组织损伤。在吞噬细胞中有效积累可激活的光敏剂（PSs）以实现细菌的选择性成像和按需光动力消融，对于精确的细菌诊断和治疗具有重要的科学和实际意义。在此，引入了 HClO 可激活的治疗诊断纳米探针 DTF-FFP NP，用于吞噬细胞中的图像引导细菌消融。DTF-FFP NPs 是通过 HClO 响应的近红外分子 FFP 和具有聚集诱导发射特性的有效 PS DTF 的纳米沉淀制备的，使用两亲聚合物 Pluronic F127 作为封装基质。作为能量受体，FFP 可以淬灭 DTF 的荧光和活性氧 (ROS) 的产生，从而消除 DTF-FFP NPs 在正常细胞和组织中的光毒性。一旦被递送到感染部位，DTF-FFP NPs 会发出红色荧光，并由于吞噬细胞中 HClO 的刺激释放而降解 FFP，从而有效地产生 ROS。体外和体内结果都成功证实了荧光和光敏的选择性激活，证明了 DTF-FFP NPs 在精确细菌治疗中的有效性和治疗诊断潜力。DTF-FFP NPs 发出红色荧光，并由于吞噬细胞中 HClO 的刺激释放而降解 FFP，从而有效地产生 ROS。体外和体内结果都成功证实了荧光和光敏的选择性激活，证明了 DTF-FFP NPs 在精确细菌治疗中的有效性和治疗诊断潜力。DTF-FFP NPs 发出红色荧光，并由于 FFP 被吞噬细胞中 HClO 的刺激释放而降解，从而有效地产生 ROS。体外和体内结果都成功证实了荧光和光敏的选择性激活，证明了 DTF-FFP NPs 在精确细菌治疗中的有效性和治疗诊断潜力。

更新日期：2020-11-25

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