The nitrogen removal, microbial communities and enzymatic activities of sequencing batch reactors (SBRs) had been compared under long-term exposure of different hydroxyl-functionalized multi-walled carbon nanotubes (MWCNTs-OH) concentrations. Compared with 0 mg/L MWCNTs-OH, the chemical oxygen demand (COD) and nitrogen removals had no obvious change at 10 and 30 mg/L MWCNTs-OH during the whole operation process. However, the microbial oxygen uptake rate, nitrifying rate, denitrifying rate, dehydrogenase activity, nitrifying enzymatic activity and denitrifying enzymatic activity decreased with the increase of MWCNTs-OH concentration and operation time owing to the toxicity of MWCNTs-OH. The microbial reactive oxygen species production and lactate dehydrogenase release under MWCNTs-OH stress kept an ascending trend as the operation time increased, which might affect the normal microbial functions. The presence of MWCNTs-OH varied the microbial community structure of SBR. The relative abundances of Nitrosomonas, Nitrosospira, Nitrospira and denitrifying genera displayed some evident changes under MWCNTs-OH stress.

在长期暴露于不同羟基官能化的多壁碳纳米管（MWCNTs-OH）浓度下，对测序间歇反应器（SBR）的脱氮，微生物群落和酶活性进行了比较。与0 mg / L MWCNTs-OH相比，在整个操作过程中，化学需氧量（COD）和氮去除量在10和30 mg / L MWCNTs-OH上没有明显变化。然而，由于MWCNTs-OH的毒性，微生物的吸氧率，硝化率，反硝化率，脱氢酶活性，硝化酶活性和反硝化酶活性随MWCNTs-OH浓度和操作时间的增加而降低。MWCNTs-OH胁迫下微生物活性氧的产生和乳酸脱氢酶的释放随着操作时间的增加而保持上升趋势，这可能会影响正常的微生物功能。MWCNTs-OH的存在改变了SBR的微生物群落结构。的相对丰度亚硝化，Nitrosospira，硝化和反硝化属显示下的MWCNT-OH应力一些明显的变化。