Abstract Entada africana (Mimosaceae) is widely used in African traditional medicine as a wound healing agent. This study investigated the wound healing, antimicrobial and anti-oxidant activities of the leaf and stem bark of E. africana in order to scientifically validate its wound healing properties. Methanol extracts of the leaf and stem bark were formulated into creams (5–20%) and tested in the dermal excision wound model in rats. The broth dilution assay was used to determine the antimicrobial activity; DPPH free radical scavenging, ferric reducing power, total antioxidant capacity, total phenolic and total flavonoid content were determined to assess the antioxidant activity. Significant wound healing was demonstrated by topical application of the extract creams. By the 10th day, a total wound surface closure of 98.8%, 95.8%, 96.3% and 73.9% was observed for the 10% stem bark and leaf creams, standard drug and blank cream treated groups respectively. Histopathological examination of the healed tissue sections showed significant collagen production, scanty inflammatory cell infiltrates and re-epithelialization whereas tissues from the blank cream treated group displayed focal areas of abscess and diffused inflammatory cells. The methanol stem bark extract demonstrated antibacterial activity against S. aureus and S. pyogenes with a minimum inhibitory concentration of 1.56 mg/mL. The stem bark and leaf had antioxidant activity and were found to contain a total phenolic content of 47.25 and 133.7 mg/g Gallic acid equivalent respectively and total flavonoid content of 21.06 and 88.21 mg/g quercetin equivalent respectively. The above results indicate that the leaves and stem bark of E. africana have great potential for the treatment of open wounds.

中文翻译：

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Entada Africana Guill 叶和茎皮的伤口愈合、抗菌和抗氧化特性。＆佩尔。

摘要 非洲菊（含羞草科）在非洲传统医学中被广泛用作伤口愈合剂。本研究调查了非洲桉叶和茎皮的伤口愈合、抗菌和抗氧化活性，以科学地验证其伤口愈合特性。将叶和茎皮的甲醇提取物配制成乳膏（5-20​​%），并在大鼠皮肤切除伤口模型中进行测试。肉汤稀释试验用于确定抗菌活性；测定DPPH自由基清除能力、铁还原能力、总抗氧化能力、总酚和总黄酮含量以评估抗氧化活性。提取物乳膏的局部应用证明了显着的伤口愈合。到第 10 天，总创面闭合率分别为 98.8%、95.8%、96.3% 和 73。对于 10% 茎皮和叶乳膏、标准药物和空白乳膏处理组分别观察到 9%。愈合组织切片的组织病理学检查显示显着的胶原蛋白产生、少量炎性细胞浸润和再上皮化，而空白乳膏治疗组的组织显示脓肿和弥漫性炎性细胞的焦点区域。甲醇茎皮提取物显示出对金黄色葡萄球菌和化脓性链球菌的抗菌活性，最低抑制浓度为 1.56 mg/mL。茎皮和叶具有抗氧化活性，发现总酚含量分别为 47.25 和 133.7 毫克/克没食子酸当量，总黄酮含量分别为 21.06 和 88.21 毫克/克槲皮素当量。以上结果表明，E.的叶和茎皮。