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Synthesis and ultrastructural observation of arbutoid mycorrhizae of black truffles ( Tuber melanosporum and T. aestivum )
Mycorrhiza ( IF 3.9 ) Pub Date : 2020-08-24 , DOI: 10.1007/s00572-020-00985-5
Francesca Ori , Marco Leonardi , Antonella Faccio , Fabiano Sillo , Mirco Iotti , Giovanni Pacioni , Raffaella Balestrini

Arbutus unedo (the strawberry tree) is a Mediterranean shrub which forms arbutoid mycorrhizae with a variety of Asco- and Basidiomycetes. After the discovery of the mycorrhizal symbiosis between A. unedo and Tuber borchii, in this study, arbutoid mycorrhizae were synthetized in greenhouse with Tuber aestivum and Tuber melanosporum. Six months after inoculation, both species colonized the roots of all inoculated A. unedo seedlings, but mature mycorrhizae were only observed after 12 months. Ultrastructure analysis of Tuber arbutoid mycorrhizae was described for the first time, showing, as observed in typical endosymbiosis, a rearrangement of host cells and the creation of an interface compartment with both truffle species. Immunolabelling experiments suggested that pectins are not present in the interface matrix surrounding the intracellular hyphae. Thus, the ability to establish symbiosis with A. unedo seems to be a common feature in the genus Tuber, opening up the possibility to use this plant for mycorrhization with valuable truffles. This could represent an important economic opportunity in Mediterranean areas by combining the production of truffles, edible fruits and valued honey.



中文翻译:

黑松露(块茎和小麦)的合成及超微结构观察

乌头杨梅(草莓树)是一种地中海灌木,可与各种亚科和担子菌形成杨梅菌根。之间的共生菌根的发现之后A. unedo块茎勃,在这项研究中,浆果莓类菌根是在温室synthetized块茎小麦块茎melanosporum。接种六个月后,这两个物种都在所有接种的阿尼多拉幼苗的根部定植,但仅在12个月后才观察到成熟的菌根。块茎的超微结构分析首次描述了arbutoid菌根,显示出在典型的共生中所观察到的,宿主细胞的重排以及与两种松露菌种的界面区室的形成。免疫标记实验表明,果胶在细胞内菌丝周围的界面基质中不存在。因此,与块茎农杆菌共生的能力似乎是块茎属的共同特征,这为将这种植物用于有价值的松露菌根的开辟了可能性。通过将松露,食用水果和珍贵蜂蜜的生产结合起来,这可能是地中海地区的重要经济机会。

更新日期:2020-10-20
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