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Effect of Hydrogen Peroxide on Plant Growth, Photosynthesis, Leaf Histology and Rubisco Gene Expression of the Ficus deltoidea Jack Var. deltoidea Jack
Journal of Plant Growth Regulation ( IF 4.8 ) Pub Date : 2020-10-20 , DOI: 10.1007/s00344-020-10243-9
Nik Nurnaeimah Nik Muhammad Nasir , Mohammad Moneruzzaman Khandaker , Khamsah Suryati Mohd , Noor Afiza Badaluddin , Normaniza Osman , Nashriyah Mat

The present study was carried out to investigate the effects of hydrogen peroxide (H2O2) on the growth, photosynthesis, leaf histology, mineral uptake, phytochemical accumulation, and Rubisco gene expression in Ficus deltoidea var. deltoidea, a slow growing medicinal plant. The F. deltoidea plants were spray-treated with 0 (control), 8, 16, 30 and 60 mM H2O2 under field conditions. The results showed that the applications of 30 mM H2O2 increased the plant height, leaf number, and syconium number by 26%, 9% and 2275%, respectively, over the control specimen. Photosynthetic rates, transpiration, stomatal conductance and chlorophyll content of F. deltoidae plants increased significantly after treatment of 30 mM H2O2. A lower concentration (8 and 16 mM) of H2O2 also improved the leaf histological properties such as tracheary elements and other vascular cells. In addition, treatments with 30 mM H2O2 increased calcium, potassium, total phenolic and flavonoids content, in F. deltoidea plants, by 14%, 15%, 12% and 72%, respectively. In addition, H2O2 treatment at 30 mM concentration downregulated the Rubisco gene with a between 1 and 5 times fold gene expression change. From this study, it can be concluded that spraying with 30 and 16 mM of H2O2 once a week enhanced growth, photosynthesis, mineral accumulation, phenol and flavonoid content, as well as aided Rubisco gene expression, under field conditions.

中文翻译:

过氧化氢对 Ficus deltoidea Jack Var 植物生长、光合作用、叶片组织学和 Rubisco 基因表达的影响。三角洲杰克

本研究旨在研究过氧化氢 (H2O2) 对 Ficus deltoidea var. 的生长、光合作用、叶片组织学、矿物质吸收、植物化学物质积累和 Rubisco 基因表达的影响。deltoidea,一种生长缓慢的药用植物。在田间条件下用 0(对照)、8、16、30 和 60 mM H 2 O 2 喷雾处理 F. deltoidea 植物。结果表明,与对照样品相比,施用 30 mM H2O2 使株高、叶数和孢子菌数分别增加了 26%、9% 和 2275%。F. deltoidae 植物的光合速率、蒸腾作用、气孔导度和叶绿素含量在 30 mM H2O2 处理后显着增加。较低浓度(8 和 16 mM)的 H2O2 也改善了叶片的组织学特性,例如气管元件和其他维管细胞。此外,用 30 mM H2O2 处理使 F. deltoidea 植物中的钙、钾、总酚类和黄酮类含量分别增加 14%、15%、12% 和 72%。此外,30 mM 浓度的 H2O2 处理下调了 Rubisco 基因,基因表达改变了 1 到 5 倍。从这项研究中可以得出结论,在田间条件下,每周喷洒 30 和 16 mM H2O2 可促进生长、光合作用、矿物质积累、苯酚和类黄酮含量,并有助于 Rubisco 基因表达。分别为 12% 和 72%。此外,30 mM 浓度的 H2O2 处理下调了 Rubisco 基因,基因表达改变了 1 到 5 倍。从这项研究中可以得出结论,在田间条件下,每周喷洒 30 和 16 mM H2O2 可促进生长、光合作用、矿物质积累、苯酚和类黄酮含量,并有助于 Rubisco 基因表达。分别为 12% 和 72%。此外,30 mM 浓度的 H2O2 处理下调了 Rubisco 基因,基因表达改变了 1 到 5 倍。从这项研究中可以得出结论,在田间条件下,每周喷洒 30 和 16 mM H2O2 可促进生长、光合作用、矿物质积累、苯酚和类黄酮含量,并有助于 Rubisco 基因表达。
更新日期:2020-10-20
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