Fusarium wilt race 1, caused by Fusarium oxysporum f. sp. lactucae, is one of the main diseases of lettuce in tropical and subtropical regions, including under summer conditions in Japan. Resistant lettuce cultivars have shown symptoms on occasion, which have led breeding programs to focus on the improvement of resistance phenotypes to race 1. Disease assays using Fusarium wilt race 1 were performed, and the lettuce cultivar ‘VI185’ was categorized as highly resistant, which was validated by subsequent population analyses. F2 individuals obtained from a cross between ‘VI185’ (highly resistant) and ‘ShinanoGreen’ (partially resistant) were analyzed, and an F3 population was subjected to an infection assay using the race 1 pathogen. ddRAD-seq analysis revealed two significant QTLs on LG7 (qFOL7.1) and LG8 (qFOL8.1) that accounted for the race 1 resistance phenotype. Furthermore, the loci that conferred elevated resistance originated from ‘VI185’ at both QTLs. Our results provide a foundation for understanding the QTLs for resistance to Fusarium wilt race 1 and provide evidence that genetic diversity is available for enhancing the current resistance in existing lettuce cultivars.

中文翻译：

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鉴定莴苣 (Lactuca sativa L.) 抗枯萎病菌 1 的两个 QTL

枯萎病小种 1，由尖孢镰刀菌 f.引起。sp. 莴苣是热带和亚热带地区生菜的主要病害之一，包括在日本的夏季条件下。抗性生菜品种有时会出现症状，这导致育种计划将重点放在改善对小种 1 的抗性表型上。使用枯萎病 1 种进行病害分析，生菜品种“VI185”被归类为高抗性，这通过随后的人口分析进行了验证。分析了从“VI185”（高度抗性）和“ShinanoGreen”（部分抗性）之间杂交获得的 F2 个体，并使用种族 1 病原体对 F3 种群进行了感染测定。ddRAD-seq 分析揭示了 LG7 (qFOL7.1) 和 LG8 (qFOL8.1) 上的两个重要 QTL。1) 这说明了种族 1 的抗性表型。此外，赋予高抗性的基因座起源于两个 QTL 的“VI185”。我们的结果为了解对枯萎病 1 号抗性的 QTL 提供了基础，并提供了证据表明遗传多样性可用于增强现有生菜品种的当前抗性。