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A DNA barcode library for ground beetles of Germany: the genus Agonum Bonelli, 1810 (Insecta, Coleoptera, Carabidae)
Deutsche Entomologische Zeitschrift ( IF 1.3 ) Pub Date : 2020-10-19 , DOI: 10.3897/dez.67.56163
Michael J. Raupach , Karsten Hannig , Jérome Morinière , Lars Hendrich

The ground beetle genus Agonum Bonelli, 1810 is a large genus of the tribe Platynini with many species that show high amounts of intraspecific variations, making a correct identification challenging. As part of the German Barcode of Life initiative, this publication provides a comprehensive DNA barcode library for species of Agonum that are reported for Germany. In total, DNA barcodes from 258 beetles and 23 species were analysed using the Barcode of Life Data System (BOLD) workbench, including sequences from former studies and 68 newly-generated sequences. The neighbour-joining analyses, based on K2P distances, revealed distinct clustering for all studied species, with unique Barcode Index Numbers (BINs) for 15 species (65%). BIN sharing but distinct clustering was found for three species pairs: Agonum micans/Agonum scitulum, Agonum impressum/Agonum sexpunctatum and Agonum duftschmidi/Agonum emarginatum. The given dataset and its analysis represent another important step in generating a comprehensive DNA barcode library for the ground beetles of Germany and Central Europe in terms of modern biodiversity research.

中文翻译:

德国地面甲虫的DNA条码文库:Agonum Bonelli属,1810年(Insecta,鞘翅目,腕足科)

地面甲虫属Agonum Bonelli属(1810)是Platynini部落的大型属,许多物种显示出大量种内变异,因此正确鉴定具有挑战性。作为《德国生命条形码》计划的一部分,该出版物为德国报道的A属物种提供了一个全面的DNA条形码库。总共,使用生命条形码系统(BOLD)工作台分析了258种甲虫和23种物种的DNA条形码,包括以前研究的序列和68条新生成的序列。基于K2P距离的邻居加入分析显示了所有研究物种的独特聚类,其中15种(65%)具有唯一的条形码索引号(BINs)。BIN共享,但发现了三个物种对的独特聚类:gon虫//虫,印/ Agonum sexpunctatum和Agonum duftschmidi / Agonum emarginatum。在现代生物多样性研究方面,给定的数据集及其分析代表了为德国和中欧的地面甲虫生成全面的DNA条形码库的又一重要步骤。
更新日期:2020-10-19
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