Temperature-activated PRP–cryogel for long-term osteogenesis of adipose-derived stem cells to promote bone repair,Materials Chemistry Frontiers

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Temperature-activated PRP–cryogel for long-term osteogenesis of adipose-derived stem cells to promote bone repair
Materials Chemistry Frontiers ( IF 6.0 ) Pub Date : 2020-10-19 , DOI: 10.1039/d0qm00493f
Xiaoliang Zhao _{1,

2,

3,

4,

5} , Huiyong Xu _{5,

6,

7,

8,

9} , Genlan Ye _{1,

2,

3,

4,

5} , Chuangkun Li _{1,

2,

3,

4,

5} , Leyu Wang _{1,

2,

3,

4,

5} , Fang Hu _{1,

2,

3,

4,

5} , Xiaozhong Qiu _{1,

2,

3,

4,

5}

Affiliation

Platelet-rich plasma (PRP) is widely used for wound healing with fairly good biocompatibility. However, it suffers from the burst release of growth factors as well as the activation with exogenous activators. In this study, we encapsulated PRP in a cryogel to address both these issues. As the cryogel was prepared in a subzero condition and could load PRP with tough covalent-bond crosslinkers, the activity of the autologous activators in the pristine PRP could be effectively saved and the release rate of the growth factors could be effectively reduced. At 37 °C, the remaining autologous activators could activate PRP without the need of exogenous activators to continuously release growth factors for more than 24 days. The prepared PRP–cryogel could then promote the long-term osteogenesis of adipose-derived stem cells (ADSCs). As a bone tissue engineering strategy, the combination of PRP, cryogel, and ADSCs provides temperature-activated PRP and slow-release growth factors for the long-term osteogenesis of ADSCs to enhance bone repair in rats.

中文翻译：

温度激活的PRP-cryogel可用于脂肪干细胞的长期成骨作用，促进骨修复

富含血小板的血浆（PRP）具有良好的生物相容性，被广泛用于伤口愈合。然而，它遭受生长因子的突然释放以及外源性激活剂的激活。在这项研究中，我们将PRP封装在冷冻凝胶中以解决这两个问题。由于冷冻凝胶是在低于零温度的条件下制备的，并且可以使PRP带有强硬的共价键交联剂，因此可以有效地保存原始PRP中自体活化剂的活性，并可以有效地降低生长因子的释放速率。在37°C时，其余的自体激活剂可以激活PRP，而无需外源性激活剂连续释放生长因子超过24天。然后，准备的PRP-cryogel可以促进脂肪干细胞（ADSCs）的长期成骨作用。

更新日期：2020-11-03

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