This study aims to screen the male human papillomavirus (HPV) infection status and genotyping in Qingcheng District, Qingyuan City, Guangdong Province, China to provide a reference basis for formulating prevention strategies for HPV infection. The present study collected urethral epithelium or scraped penile epidermis from high-risk male patients in Qingyuan People's Hospital during the last five years, extracted DNA fragments using the boiling method, and detected 23 types of HPV genotypes by PCR-reverse blot hybridization. The positive detection rate was 54.31% of 1044 males with high risk of HPV (567/1044). Among these males, the positive detection rate of HPV was the highest in patients initially diagnosed with warts, and the rate was 66.47%. Five main HPV types are identified as follows: HPV6 18.87% (197/1044), HPV11 10.25% (107/1044), HPV52 8.81% (92/1044), HPV16 6.90% (72/1044), and HPV51 5.08% (53/1044). Among these HPV-infected patients, single infection mainly by low-risk HPV6 and HPV11 accounted for 56.61% (321/567); high- and low-risk combined HPV co-infections accounted for 29.10% (165/567). The HPV infected patients was mainly between 21 and 40 years old, and the HPV infection rate was higher with increased age. The HPV infection rate in the Qingyuan area is higher than in other areas and the main infection is single infection. Furthermore, HPV52, HPV16, and HPV51 are the main high-risk infection types, while HPV6 and HPV11 are the main low-risk infection types.

中文翻译：

---

清远地区男性乳头瘤病毒感染及基因分型

本研究旨在筛查广东省清远市清城区男性人乳头瘤病毒（HPV）感染状况及基因分型，为制定HPV感染预防策略提供参考依据。本研究收集清远市人民医院近五年高危男性患者尿道上皮或刮除的阴茎表皮，采用煮沸法提取DNA片段，采用PCR-反向印迹杂交法检测出23种HPV基因型。1044名HPV高危男性（567/1044）的阳性检出率为54.31%。这些男性中，初诊尖锐湿疣患者HPV阳性检出率最高，达66.47%。五种主要 HPV 类型确定如下：HPV6 18.87% (197/1044)，HPV11 10.25% (107/1044)，HPV52 8.81% (92/1044)、HPV16 6.90% (72/1044) 和 HPV51 5.08% (53/1044)。在这些HPV感染患者中，以低危型HPV6和HPV11为主的单一感染占56.61%（321/567）；高危和低危合并HPV合并感染占29.10%（165/567）。HPV感染者主要集中在21～40岁之间，且随着年龄的增长HPV感染率较高。清远地区HPV感染率高于其他地区，以单一感染为主。此外，HPV52、HPV16和HPV51是主要的高危感染类型，而HPV6和HPV11是主要的低危感染类型。高危和低危合并HPV合并感染占29.10%（165/567）。HPV感染者主要集中在21～40岁之间，且随着年龄的增长HPV感染率较高。清远地区HPV感染率高于其他地区，以单一感染为主。此外，HPV52、HPV16和HPV51是主要的高危感染类型，而HPV6和HPV11是主要的低危感染类型。高危和低危合并HPV合并感染占29.10%（165/567）。HPV感染患者主要集中在21～40岁之间，且随着年龄的增长HPV感染率较高。清远地区HPV感染率高于其他地区，以单一感染为主。此外，HPV52、HPV16和HPV51是主要的高危感染类型，而HPV6和HPV11是主要的低危感染类型。