To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

中文翻译：

---

新型产油酵母太平洋红酵母INDKK利用金黄壳水解物聚合甘油三酯

为了满足当前的运输需求并解决食物与燃料的问题，微生物脂质衍生的生物燃料正受到全世界的关注。本研究的重点是通过微波辅助尼罗红荧光分光光度法对含油酵母进行高通量筛选，并探索将红薯壳水解物 (PSH) 作为使用新型含油酵母 Rhodotorula pacifica INDKK 生产脂质的原料。鉴定并选择了一种新的产油酵母 R. pacifica INDKK 用于微生物脂质生产。R. pacifica INDKK 在生长 120 小时后产生最大 12.8 ± 0.66 g/L 的干细胞重量和 6.78 ± 0.4 g/L 的脂质滴度，对预处理衍生的抑制剂如 5-羟甲基糠醛（5 -HMF)、(2 g/L)、糠醛 (0.5 g/L) 和乙酸 (0.5 g/L)，以及同化 C3、C5 和 C6 糖的能力。有趣的是，R. 与酸处理的解毒 PSH (AD-PSH) (0.037 ± 0.006 g/L/h) 相比，pacifica INDKK 在碱处理的糖化 PSH (AS-PSH) (0.058 ± 0.006 g/L/h) 中生长时表现出更高的脂质积累h) 和 YNB 培养基 (0.055 ± 0.003 g/L/h)。主要脂肪酸成分是油酸、棕榈酸、亚油酸和亚麻酸，估计十六烷值 (CN) 约为 56.7，表明燃料质量良好。这些结果表明，PSH 和 R. pacifica INDKK 可被视为可持续生物柴油生产的潜在原料。估计十六烷值 (CN) 约为 56.7 的亚油酸和亚麻酸，表明燃料质量良好。这些结果表明，PSH 和 R. pacifica INDKK 可被视为可持续生物柴油生产的潜在原料。估计十六烷值 (CN) 约为 56.7 的亚油酸和亚麻酸，表明燃料质量良好。这些结果表明，PSH 和 R. pacifica INDKK 可被视为可持续生物柴油生产的潜在原料。