Plants display sophisticated mechanisms to tolerate challenging environmental conditions and need to manage their ontogenesis in parallel. Here, we set out to generate an RNA-Seq time series dataset throughout grapevine (Vitis vinifera) early bud development. The expression of the developmental regulator VviAP1 served as an indicator for progress of development. We investigated the impact of changing temperatures on gene expression levels during the time series and detected a correlation between increased temperatures and a high expression level of genes encoding heat-shock proteins. The data set also allowed the exemplary investigation of expression patterns of genes from three transcription factor (TF) gene families, namely MADS-box, WRKY, and R2R3-MYB genes. Inspection of the expression profiles from all three TF gene families indicated that a switch in the developmental program takes place in July which coincides with increased expression of the bud dormancy marker gene VviDRM1.

中文翻译：

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葡萄芽发育的RNA-Seq时间序列揭示了表达模式与局部温度曲线的相关性

植物显示出可以耐受具有挑战性的环境条件的复杂机制，并且需要并行管理其本体发生。在这里，我们着手在整个葡萄（Vitis vinifera）早期芽发育过程中生成一个RNA-Seq时间序列数据集。发育调节因子VviAP1的表达作为发展进度的指标。我们调查了温度变化在时间序列中对基因表达水平的影响，并检测到温度升高和编码热激蛋白的基因的高表达水平之间的相关性。该数据集还允许示例性地研究来自三个转录因子（TF）基因家族（即MADS-box，WRKY和R2R3-MYB基因）的基因的表达模式。检查所有三个TF基因家族的表达谱表明，发育程序的转换发生在7月，这与芽休眠标记基因VviDRM1的表达增加相吻合。