MYB transcription factors (TFs) represents one of the largest TF families in plants. In this study, we performed genome-wide MYB-domain screening and identified a total of 997 MYBs in wheat (Triticum aestivum), among which 445 were 2-domain MYBs (R2R3-MYBs) that were clustered into 15 subgroups with varied conservation profiles. Homologous genes were highly conserved across the three subgenomes, with minor variations contributed by segmental duplications. Tandem and proximal gene duplications have contributed significantly to the expansion of the wheat Myb gene family. Furthermore, comprehensive transcriptome profiling of R2R3-Myb genes in 61 different tissue and time point samples revealed a clear pattern of temporal and spatial variations within six expression groups. The comprehensive genomic and transcriptional analyses provided valuable insights into the evolution and biological functions of R2R3-Myb genes in wheat. They would serve as a useful guide to further investigate the potential agronomic traits controlled by this large TF family.

中文翻译：

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小麦R2R3-MYB基因家族的全基因组鉴定和转录分析

MYB转录因子（TFs）代表植物中最大的TF家族之一。在这项研究中，我们进行了全基因组MYB结构域筛选，并鉴定了小麦（Triticum aestivum）中的997个MYB，其中445个是2结构域MYB（R2R3-MYB），它们被分为15个具有不同保护谱的亚组。 。同源基因在三个亚基因组中高度保守，节段重复贡献较小。串联和近端基因重复显着促进​​了小麦Myb基因家族的扩展。此外，在61个不同的组织和时间点样本中，R2R3-Myb基因的完整转录组谱分析揭示了六个表达组内时空变化的清晰模式。全面的基因组和转录分析为小麦R2R3-Myb基因的进化和生物学功能提供了宝贵的见识。它们将作为进一步研究由这个大TF家族控制的潜在农艺性状的有用指南。