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Rapid Differential Diagnosis of Seven Human Respiratory Coronaviruses Based on Centrifugal Microfluidic Nucleic Acid Assay
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-10-19 , DOI: 10.1021/acs.analchem.0c03364 Huiwen Xiong 1 , Xin Ye 1 , Yang Li 2 , Lijuan Wang 2 , Jin Zhang 3 , Xueen Fang 1 , Jilie Kong 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2020-10-19 , DOI: 10.1021/acs.analchem.0c03364 Huiwen Xiong 1 , Xin Ye 1 , Yang Li 2 , Lijuan Wang 2 , Jin Zhang 3 , Xueen Fang 1 , Jilie Kong 1
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With the global outbreak of the coronavirus disease 2019 (COVID-19), the highly infective, highly pathogenic, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has attracted great attention. Currently, a method to simultaneously diagnose the seven known types human coronaviruses remains lacking and is urgently needed. In this work, we successfully developed a portable microfluidic system for the rapid, accurate, and simultaneous detection of SARS-CoV, middle east respiratory syndrome coronavirus (MERS-CoV), SARS-CoV-2, and four other human coronaviruses (HCoVs) including HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1. The disk-like microfluidic platform integrated with loop-mediated isothermal amplification provides highly accurate, sensitive, and specific results with a wide linear range within 40 min. The diagnostic tool achieved 100% consistency with the “gold standard” polymerase chain reaction in detecting 54 real clinical samples. The integrated system, with its simplicity, is urgently needed for the diagnosis of SARS-CoV-2 during the COVID-19 pandemic.
中文翻译：
基于离心微流控核酸检测的七种人类呼吸道冠状病毒的快速鉴别诊断
随着2019冠状病毒病（COVID-19）在全球爆发，高传染性、高致病性的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起了高度关注。目前，尚缺乏同时诊断已知七种人类冠状病毒的方法，亟需。在这项工作中，我们成功开发了一种便携式微流控系统，用于快速、准确、同时检测 SARS-CoV、中东呼吸综合征冠状病毒 (MERS-CoV)、SARS-CoV-2 和其他四种人类冠状病毒 (HCoV)包括 HCoV-229E、HCoV-OC43、HCoV-NL63 和 HCoV-HKU1。与环介导等温扩增集成的盘状微流控平台可在 40 分钟内提供高精度、灵敏且特异的结果，并具有宽线性范围。该诊断工具在检测54个真实临床样本时，与“金标准”聚合酶链反应达到了100%的一致性。该集成系统因其简单性而迫切需要在 COVID-19 大流行期间诊断 SARS-CoV-2。
更新日期：2020-11-03
中文翻译：
基于离心微流控核酸检测的七种人类呼吸道冠状病毒的快速鉴别诊断
随着2019冠状病毒病（COVID-19）在全球爆发，高传染性、高致病性的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起了高度关注。目前，尚缺乏同时诊断已知七种人类冠状病毒的方法，亟需。在这项工作中，我们成功开发了一种便携式微流控系统，用于快速、准确、同时检测 SARS-CoV、中东呼吸综合征冠状病毒 (MERS-CoV)、SARS-CoV-2 和其他四种人类冠状病毒 (HCoV)包括 HCoV-229E、HCoV-OC43、HCoV-NL63 和 HCoV-HKU1。与环介导等温扩增集成的盘状微流控平台可在 40 分钟内提供高精度、灵敏且特异的结果，并具有宽线性范围。该诊断工具在检测54个真实临床样本时，与“金标准”聚合酶链反应达到了100%的一致性。该集成系统因其简单性而迫切需要在 COVID-19 大流行期间诊断 SARS-CoV-2。
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