Whole genome sequencing has become a powerful tool in modern microbiology. Especially bacterial genomes are sequenced in high numbers. Whole genome sequencing is not only used in research projects, but also in surveillance projects and outbreak investigations. Many whole genome analysis workflows begins with the production of a genome assembly. To accomplish this, a number of different sequencing technologies and assembly methods are available. Here, a summarization is provided over the most frequently used sequence technology and genome assembly approaches reported for the bacterial RefSeq genomes and for the bacterial genomes submitted as belonging to a surveillance project. The data is presented both in total and broken up on a per year basis. Information associated with over 400,000 publically available genomes dated April 2020 and prior were used. The information summarized include (i) the most frequently used sequencing technologies, (ii) the most common combinations of sequencing technologies, (iii) the most reported sequencing depth, and (iv) the most frequently used assembly software solutions. In all, this mini review provides an overview of the currently most common workflows for producing bacterial whole genome sequence assemblies.

全基因组测序已成为现代微生物学中的强大工具。特别是细菌基因组被大量测序。全基因组测序不仅用于研究项目，而且还用于监视项目和暴发调查。许多全基因组分析工作流程始于基因组装配的生产。为此，可以使用多种不同的测序技术和组装方法。在此，对细菌RefSeq基因组和提交为监视项目的细菌基因组的最常用序列技术和基因组组装方法进行了概述。数据以总计形式显示，并且每年细分。与400多个相关的信息，使用了2020年4月之前的000个可公开获得的基因组。汇总的信息包括（i）最常用的测序技术，（ii）测序技术的最常见组合，（iii）报道最多的测序深度，以及（iv）最常用的组装软件解决方案。总而言之，这篇小型综述概述了当前最常见的细菌全基因组序列装配生产流程。