Screening natural products for bacteriocin-producing bacteria may be the equilibrium point between the consumer demand for mild processing and the industry’s need for hazard control. Raw unprocessed honeycombs filled with oregano honey from the alpine mountainous territory of Epirus, Greece were screened for bacteriocinogenic lactic acid bacteria and Bifidobacterium spp., with inhibitory action towards some pathogens and spoilage microorganisms isolated from fresh fruits and vegetables (number and type of strains: three E. coli, twoL. monocytogenes, two Salmonella spp., two B.cereus, two Erwinia spp., one Xanthomonas spp., L. innocua (ATCC 33090TM) and E. coli 0157:H7 (ATCC 69373)). Among the 101 collected isolates (73 Lactobacillus, 8 Lactococcus, 8 Leuconostoc and 12 Bifidobacterium species) from the oregano honeycombs (an original finding since there are no other reports on the microbial biodiversity of the flora of the oregano honey), 49 strains of lactic acid bacteria (LAB) and Bifidobacterium spp. were selected and tested for their bacteriocin-producing capacity (34 Lactobacillus, 6 Lactococcus, 5 Leuconostoc and 4 Bifidobacterium). The antibacterial activity exerted by the tested LAB and Bifidobacterium strains was not of the same potency. Our results suggest that the main molecules involved in the antimicrobial activity are probably bacteriocin-like substances (a conclusion based on reduced antibacterial activity after the proteolytic treatment of the cell-free supernatant of the cultures) and this antimicrobial activity is specific for the producing strains as well as for the target strains. The spoilage bacteria as well as the reference microorganisms showed increased resistance to the bacteriocin-like substances in comparison to the wild-type pathogens.

中文翻译：

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从蜂窝中分离出的产细菌素的乳酸菌和双歧杆菌对水果和蔬菜中变质微生物和病原菌的抵抗力

筛选天然产物中产生细菌素的细菌可能是消费者对温和加工的需求与行业对危害控制的需求之间的平衡点。对来自希腊伊庇鲁斯高山地区的牛至蜂蜜的未经加工的未经加工的蜂窝状蜂窝进行了筛选，以检测细菌致癌的乳酸菌和双歧杆菌属菌种，对从新鲜水果和蔬菜中分离出来的某些病原体和腐败微生物具有抑制作用（菌株的数量和类型： 3个大肠杆菌，2个单核细胞增生李斯特菌，2个沙门氏菌，2个蜡状芽孢杆菌，2个欧文氏菌，1个黄单胞菌属，无害李斯特菌（ATCC 33090TM）和大肠杆菌0157：H7（ATCC 69373））。在从牛至蜂窝中收集的101种分离株中（73种乳酸杆菌，8个乳球菌，8个亮双歧杆菌和12个双歧杆菌）（一个原始发现，因为没有其他有关牛至蜂蜜菌群的微生物多样性的报道），有49株乳酸菌酸性细菌（LAB）和双歧杆菌属。选择并测试它们产生细菌素的能力（34个乳杆菌，6个乳球菌，5个亮粘菌和4个双歧杆菌）。被测LAB和双歧杆菌发挥的抗菌活性菌株的效力不同。我们的结果表明，涉及抗菌活性的主要分子可能是细菌素样物质（基于对蛋白的无细胞上清液进行蛋白水解处理后抗菌活性降低的结论），这种抗菌活性对生产菌株具有特异性以及目标菌株。与野生型病原体相比，腐败细菌和参考微生物对细菌素样物质的抵抗力增强。