The aim of this study was to determine whether and how poultry litter compost and dairy manure compost alter the microbial communities within field soils planted with spinach. In three successive years, separate experimental plots on two fields received randomly assigned compost treatments varying in animal origin: dairy manure (DMC), poultry litter (PLC), or neither (NoC). The composition and function of bacterial and fungal communities were characterized by the amplicon sequencing of marker genes and by the ecoenzyme activity, respectively. The temporal autocorrelation within and among years was adjusted by principal response curves (PRC) to analyze the effect of compost on community composition among treatments. Bacteria in the phylum Bacteriodetes, classes Flavobacteriia and Spingobacteriales (Fluviicola, Flavobacteriia, and Pedobacter), were two to four times more abundant in soils amended with PLC than DMC or NoC consistently among fields and years. Fungi in the phylum Ascomycota were relatively abundant, but their composition was field-specific and without treatment differences. The ecoenzyme data verify that the effects of PLC and DMC on soil communities are based on their microbial composition and not a response to the C source or nutrient content of the compost.

中文翻译：

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细菌群落动态区分家禽堆肥与乳制品堆肥和菠菜种植的非改良土壤

这项研究的目的是确定家禽垃圾堆肥和奶牛粪肥堆肥是否以及如何改变菠菜种植的田间土壤中的微生物群落。在连续三年中，在两个田地上的单独实验地块接受了随机分配的不同动物来源的堆肥处理方法：乳肥（DMC），家禽垫料（PLC）或两者都不做（NoC）。细菌和真菌群落的组成和功能分别通过标记基因的扩增子测序和生态酶活性来表征。通过主响应曲线（PRC）调整年内和年间的时间自相关，以分析堆肥对处理之间群落组成的影响。细菌杆菌门中的细菌，黄杆菌属和Spingobacteriales类（Fluviicola，在田间和年份之间，用PLC改良的土壤中的黄杆菌和Pedobacter的含量是DMC或NoC的2至4倍。子囊门中的真菌相对丰富，但是它们的组成是田间特异性的，没有处理差异。生态酶数据证实，PLC和DMC对土壤群落的影响是基于它们的微生物组成，而不是对堆肥中C源或养分含量的响应。