Xanthomonas campestris pv. campestris (Xcc) is an important seed-borne bacterial pathogen that causes black rot in brassica. Current seed disinfection methods for Xcc have disadvantages; chemical treatment has associated environmental risks, hot water immersion reduces germination, and dry heat treatment is protracted. Here, we treated Xcc-contaminated seeds with CAC-717, a recently developed disinfectant produced by applying an electric field and water flow to distilled water containing calcium hydrogen carbonate to produce mesoscopic crystals. The decimal reduction time (D-value) of Xcc suspension (8.22 log₁₀ colony forming units (CFU)/mL) by CAC-717 treatment was 0.319 min. Treatment of Xcc-contaminated cabbage seeds at 25 °C for 30 min with CAC-717 significantly reduced bacterial cell numbers recovered from the seeds (0.36 log₁₀ CFU/mL (SEM (standard error of the mean) = 0.23 log₁₀ CFU/mL)) compared with distilled water treatment (3.52 log₁₀ CFU/mL (SEM = 0.12 log₁₀ CFU/mL)). Moreover, there was a lower incidence of black rot after treatment with CAC-717 (26.67% ± 3.33%) versus distilled water (56.67% ± 8.82%). For non-contaminated seeds, there was no significant difference in germination rate and plant stem length between distilled water and CAC-717 treatment after 5 days of cultivation. In conclusion, CAC-717 is a promising seed disinfectant without deleterious effects on germination or plant growth.

中文翻译：

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含碳酸氢钙介观晶体的带电消毒剂，作为控制野油菜黄单胞菌（xanthomonas campestris pv）的潜在措施。白菜种子上的油菜

Xanthomonas campestris pv。樟脑（Xcc）是一种重要的种子传播细菌病原体，可引起芸苔黑腐病。当前的Xcc种子消毒方法有缺点。化学处理具有相关的环境风险，热水浸泡会减少发芽，并且干热处理会很长。在这里，我们处理XCC -contaminated种子与CAC-717，通过施加电场和水流到含有碳酸氢钙，以产生介观晶体蒸馏水制备的最近开发的消毒剂。Xcc暂停的小数减少时间（D值）（8.22 log ₁₀CAC-717处理的菌落形成单位（CFU）/ mL）为0.319分钟。的治疗XCC -contaminated卷心菜种子在25℃下用CAC-717显著减少细菌细胞数目30分钟从种子（0.36日志恢复₁₀ CFU /毫升（SEM（平均值的标准误差的）= 0.23日志₁₀ CFU / mL的）与蒸馏水处理（3.52 log ₁₀ CFU / mL（SEM = 0.12 log ₁₀CFU / mL））。此外，与蒸馏水（56.67％±8.82％）相比，用CAC-717处理后黑腐病的发生率较低（26.67％±3.33％）。对于无污染的种子，培养5天后，蒸馏水和CAC-717处理之间的发芽率和植物茎长没有显着差异。总之，CAC-717是一种很有前途的种子消毒剂，对发芽或植物生长没有有害影响。