Pseudomonas aeruginosa is one of the most important nosocomial pathogens and understanding its virulence is the key to effective control of P. aeruginosa infections. The regulatory network governing virulence factor production in P. aeruginosa is exceptionally complex. Previous studies have shown that the peptide chain release factor methyltransferase PrmC plays an important role in bacterial pathogenicity. Yet, the underlying molecular mechanism is incompletely understood. In this study, we used untargeted liquid and gas chromatography coupled to mass spectrometry to characterise the metabolome of a prmC defective P. aeruginosa PA14 strain in comparison with the corresponding strain complemented with prmC in trans. The comprehensive metabolomics data provided new insight into the influence of prmC on virulence and metabolism. prmC deficiency had broad effects on the endo- and exometabolome of P. aeruginosa PA14, with a marked decrease of the levels of aromatic compounds accompanied by reduced precursor supply from the shikimate pathway. Furthermore, a pronounced decrease of phenazine production was observed as well as lower abundance of alkylquinolones. Unexpectedly, the metabolomics data showed no prmC-dependent effect on rhamnolipid production and an increase in pyochelin levels. A putative virulence biomarker identified in a previous study was significantly less abundant in the prmC deficient strain.

中文翻译：

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肽链释放因子甲基转移酶PrmC影响铜绿假单胞菌PA14内和外代谢组

铜绿假单胞菌是最重要的医院病原体之一，了解其毒力是有效控制铜绿假单胞菌感染的关键。铜绿假单胞菌生产毒力因子的法规网络异常复杂。先前的研究表明，肽链释放因子甲基转移酶PrmC在细菌致病性中起重要作用。然而，潜在的分子机理尚未完全理解。在这项研究中，我们使用了非靶向液相色谱和气相色谱联用质谱技术，以鉴定prmC缺陷型铜绿假单胞菌PA14菌株的代谢组以及与之互补的相应菌株。反式prmC。全面的代谢组学数据为prmC对毒力和代谢的影响提供了新的见解。prmC缺乏症对铜绿假单胞菌PA14的内代谢和外代谢组有广泛的影响，芳香族化合物的含量显着下降，而the草酸酯途径的前体供应减少。此外，观察到吩嗪产量的显着降低以及烷基喹诺酮的较低丰度。出乎意料的是，代谢组学数据显示对鼠李糖脂产生没有prmC依赖性作用，且秋水仙素水平增加。在先前的研究中鉴定出的推定毒力生物标志物在prmC中的含量明显不足 应变不足。