In the present trial, 160 heavy-size male broiler chickens were allocated to 4 dietary treatments (control feed [C] and 5, 10 and 15% TM meal inclusion, respectively, with 5 replicate pens/treatment and 8 birds/pen) to evaluate the influence of TM meal on intestinal microbiota and mucin composition. The broiler chickens fed TM-based diets showed higher beta diversity of their cecal microbiota in comparison with the C birds (p < 0.001). A significant decrease of the relative abundance of Firmicutes phylum and lower Firmicutes:Bacteroidetes ratios (False Discovery Rate [FDR] < 0.05) were also identified in TM15 broiler chickens when compared to the C group. Furthermore, the TM birds showed decreased relative abundance of Clostridium, Coprococcus, L-Ruminococcus and Ruminococcus genera (FDR < 0.05). In relation to the gut mucin composition, higher mucin staining intensity was detected in the intestinal crypts of TM5 birds in comparison with the other TM groups (p < 0.05). In conclusion, dietary TM meal inclusion negatively influenced the cecal microbiota of heavy-size broiler chickens in terms of partial alteration of the physiological microbial population and reduction of the potential beneficial bacteria (with slightly more pronounced effects when testing the 10–15% inclusion levels).

中文翻译：

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大型肉鸡日粮中含有黄粉虫：对肠道菌群和粘蛋白动力学的影响

在本试验中，将160只大号雄性肉鸡分配到4种饮食处理中（对照饲料[C]和5、10和15％TM进餐，分别使用5支重复笔/处理和8只禽/笔）。评估TM餐对肠道菌群和粘蛋白组成的影响。与C禽相比，饲喂以TM为基础的日粮的肉鸡的盲肠微生物群具有更高的β多样性（p <0.001）。与C组相比，在TM15肉鸡中还确认到门扇菌门的相对丰度显着降低，而门扇菌与拟杆菌的比率较低（假发现率[FDR] <0.05）。此外，TM鸟显示梭状芽胞杆菌，协球菌， L-瘤胃球菌和瘤胃球菌属（FDR <0.05）。关于肠道粘蛋白成分，与其他TM组相比，在TM5禽的肠道隐窝中检测到更高的粘蛋白染色强度（p <0.05）。总而言之，饮食中的膳食粉餐对部分大规格肉鸡的盲肠微生物区系产生了不利的影响，即部分改变了生理微生物种群并减少了潜在的有益细菌（在测试10–15％的夹杂物水平时效果稍显着） ）。