Abstract

Purpose

Tumor treating fields (TTF) therapy is a noninvasive method that uses alternating electric fields to treat various types of cancer. This study demonstrates the combined effect of TTF and radiotherapy (RT) in vitro on pancreatic cancer, which is known to be difficult to treat.

Materials and methods

In CFPAC-I and HPAF-II pancreatic cancer cell lines, the combined in vitro effect of TTF and RT was evaluated by measuring cell counts, markers of apoptosis, and clonogenic cell survival. The synergy effects were verified using the Valeriote and Carpentier equations.

Results

TTF and RT inhibited cancer cell growth more effectively than did monotherapy with TTF or RT. The combined treatment also enhanced apoptosis more than monotherapy, as shown by assays for cleaved poly (ADP-ribose) polymerase (PARP) and annexin V. In addition, on the survival curve, this treatment method has been shown to work synergistically.

Conclusion

These results suggest that combined treatment with TTF and RT may be a good alternative treatment for patients with pancreatic cancer.

中文翻译：

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肿瘤治疗场（TTF）治疗可增强胰腺癌细胞的辐射诱导凋亡

摘要

目的

肿瘤治疗场（TTF）治疗是一种非侵入性方法，使用交流电场治疗各种类型的癌症。这项研究证明了TTF和放疗（RT）在体外对胰腺癌的联合作用，已知这是很难治疗的。

材料和方法

在CFPAC-I和HPAF-II胰腺癌细胞系中，TTF和RT的联合体外作用通过测量细胞计数，凋亡标记和克隆细胞存活来评估。使用Valeriote和Carpentier方程验证了协同效应。

结果

与单用TTF或RT疗法相比，TTF和RT更有效地抑制癌细胞生长。联合治疗也比单药治疗更能增强细胞凋亡，如裂解的聚（ADP-核糖）聚合酶（PARP）和膜联蛋白V的测定所示。此外，在存活曲线上，该治疗方法已显示出协同作用。

结论

这些结果表明，TTF和RT联合治疗对于胰腺癌患者可能是一个很好的替代治疗方法。