ABSTRACT

Staphylococcus aureus is a common foodborne pathogenic microorganism which can cause food poisoning and it is pathogenic to both humans and animals. Therefore, rapid detection of S. aureus infection is of great significance. In this study, a microfluidic platform was introduced to detect S. aureus by fluorescence labeling method and a self-made microfluidic chip, which has immune spheres were used to study the effect of capturing S. aureus. Through this experiment, we found that the platform can be used for microbial culture, and S. aureus antibody coated on the diameter of 50 ~ 90 μm microspheres for detection. On the premise of optimizing the sample flow rate and detection time, the bacterial detection was quantitatively monitored. Results showed that our platform can detect S. aureus at injection rate of 5 μL·min⁻¹ reacted for 4 min and the detection limit of bacteria is 1.5 × 10¹ CFU/μL. However, the detection time of traditional method is 24 hs to 72 hs, and the operation is complex and cumbersome. These findings indicated that the microfluidic chip in this study is portable, sensitive, and accurate, laying a good foundation for further research on the application of rapid bacterial detection platform.

摘要

金黄色葡萄球菌是一种常见的食源性致病微生物，可引起食物中毒，对人和动物均有致病性。因此，快速检测金黄色葡萄球菌感染具有重要意义。本研究引入微流控平台通过荧光标记法检测金黄色葡萄球菌，并利用自制的具有免疫球体的微流控芯片来研究捕获金黄色葡萄球菌的效果。通过本次实验，我们发现该平台可用于微生物培养，金黄色葡萄球菌抗体包被在直径为50～90 μm的微球上进行检测。在优化样品流速和检测时间的前提下，对细菌检测进行定量监测。结果表明，我们的平台可以以5 μL·min ^{-1 的}注射速率检测金黄色葡萄球菌，反应4 min，细菌检出限为1.5 × 10 ¹ CFU/μL。但传统方法检测时间为24~72 h，操作复杂繁琐。这些发现表明本研究中的微流控芯片具有便携、灵敏、准确的特点，为进一步研究细菌快速检测平台的应用奠定了良好的基础。