The black basses Micropterus spp. are among the most important game fishes in the United States. Translocation by both anglers and state agencies for increased angling opportunity has led to widespread hybridization between native and nonnative species. The use of species‐diagnostic single‐nucleotide polymorphism (SNP) markers for black basses has allowed for rapid assessment of species identification and hybridization in rivers and streams. Here, we report genetic evidence for the presence of Redeye Bass M. coosae and Alabama Bass M. henshalli outside of their native range and their hybridization with native species of black bass in Town Creek, a tributary of the Tennessee River. To determine which black bass species were present in the creek, four sites were sampled using both angling and backpack electrofishing. Sampled DNA from fin clips was analyzed using a diagnostic SNP panel. Genetic results confirmed that Redeye Bass and Alabama Bass were present as nonnative congeners in the creek. Interestingly, no pure individual Smallmouth Bass M. dolomieu or Spotted Bass M. punctulatus were found, although both species are native to the stream. Phenotypic identification followed by genotypic classification revealed that 62% of the fish collected for this study were misidentified in the field, with 100% of those being hybrids, indicating the importance of genetic assessment in conjunction with classic morphometrics for the management of black basses. Further studies are needed to determine whether seasonality plays a role in species presence and abundance and whether population profiles identified here are observed more broadly within the Tennessee River system.

中文翻译：

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引入的红眼鲈和阿拉巴马州鲈的遗传证据以及与天然微翅菌的杂交。在美国阿拉巴马州Town Creek

黑鲈Micropterus spp。是美国最重要的野味鱼之一。钓鱼者和国家机构的转移都增加了钓鱼的机会，从而导致了本地物种与非本地物种之间的广泛杂交。对黑鲈使用物种诊断性单核苷酸多态性（SNP）标记可以快速评估河流和河流中的物种识别和杂交。在这里，我们报告了红眼鲈M. coosae和阿拉巴马鲈M. henshalli的遗传证据在田纳西州河的支流Town Creek之外，还与本地黑鲈杂交。为了确定小溪中存在哪些黑鲈物种，使用垂钓和背包式电钓鱼对四个地点进行了采样。使用诊断SNP面板分析了从鳍夹中取样的DNA。遗传结果证实，红眼鲈和阿拉巴马鲈是小河的非本地同类物。有趣的是，没有纯粹的小嘴鲈M. dolomieu或斑点鲈M. punctulatus被发现，尽管这两个物种都是溪流的原生物种。进行表型鉴定和基因型分类后发现，在本研究中，有62％的鱼类在田间被误认，其中100％是杂种，表明遗传评估与经典形态学相结合对于黑鲈的管理非常重要。需要进一步研究以确定季节性是否在物种存在和丰度中起作用，以及在田纳西河系统中是否更广泛地观察到此处确定的种群概况。