Forty‐seven genotypes and one wild relative of soybean, Glycine soja, were screened for resistance against aerial blight under epiphytotic conditions in the field during the Kharif season of two consecutive years viz., 2016 and 2017. Out of the 48 genotypes screened, only 18 genotypes exhibited a moderately resistant response to aerial blight during both the years of study. In order to perform molecular screening of the genotypes for aerial blight resistance, the genomic DNA obtained from the seedlings of the forty‐eight soybean genotypes was subjected to PCR amplification with 12 SSR markers. The SSR markers Satt 119, Sat_076, Satt 433, Satt 281, Satt 277, Satt 245 and Satt 520 were able to clearly amplify different banding pattern for resistant and susceptible genotypes, out of which Satt 433 and Satt 520 were found to exhibit a pattern, highly similar to the results of field screening of the genotypes with respect to resistant and susceptible reaction to the disease. The eighteen soybean genotypes that exhibited moderately resistant reaction to RAB under field conditions during both the years showed a banding pattern similar to resistant check PS‐1583 in the amplification profile produced by the SSR markers. The polymorphism information content (PIC) from the analysis of amplification profile of the SSR markers used in the study, ranged from 0.58 to 0.95. The dendrogram constructed using UPGMA cluster analysis clearly differentiated the resistant and susceptible genotypes of soybean into two separate groups.

中文翻译：

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使用SSR标记评估不同基因型对空中疫病（Rhizoctonia solani Kuhn）的响应之间的遗传多样性

四十七个基因型和大豆的一个野生近缘野生大豆，在筛选针对在外地epiphytotic条件下空中白叶枯病抗性秋收季节连续两年即分别是2016年和2017年。在这两年的研究中，筛选出的48个基因型中，只有18个基因型表现出对白叶枯病的中等抗性。为了对抗空疫病的基因型进行分子筛选，将从48个大豆基因型的幼苗中获得的基因组DNA进行了12个SSR标记的PCR扩增。SSR标记Satt 119，Sat_076，Satt 433，Satt 281，Satt 277，Satt 245和Satt 520能够清楚地扩增针对抗性和易感基因型的不同条带模式，其中Satt 433和Satt 520被发现表现出一种模式，与对该病的耐药性和易感性反应的基因型的现场筛选结果高度相似。在这两年中，在田间条件下对RAB表现出中等抗性的18种大豆基因型在由SSR标记产生的扩增曲线中显示出类似于抗性检查PS-1583的条带模式。通过研究中使用的SSR标记的扩增图谱分析，多态信息含量（PIC）为0.58至0.95。使用UPGMA聚类分析构建的树状图清楚地将大豆的抗药性和易感基因型分为两个独立的组。