ANNEXIN 8 negatively regulates RPW8.1‐mediated cell death and disease resistance in Arabidopsis,Journal of Integrative Plant Biology

当前位置： X-MOL 学术 › J. Integr. Plant Biol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

ANNEXIN 8 negatively regulates RPW8.1‐mediated cell death and disease resistance in Arabidopsis
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2020-10-19 , DOI: 10.1111/jipb.13025
Zhi-Xue Zhao ₁ , Yong-Ju Xu ₁ , Yang Lei ₁ , Qin Li ₁ , Ji-Qun Zhao ₁ , Yan Li ₁ , Jing Fan ₁ , Shunyuan Xiao ₂ , Wen-Ming Wang ₁

Affiliation

Study on the regulation of broad‐spectrum resistance is an active area in plant biology. RESISTANCE TO POWDERY MILDEW 8.1 (RPW8.1) is one of a few broad‐spectrum resistance genes triggering the hypersensitive response (HR) to restrict multiple pathogenic infections. To address the question how RPW8.1 signaling is regulated, we performed a genetic screen and tried to identify mutations enhancing RPW8.1‐mediated HR. Here, we provided evidence to connect an annexin protein with RPW8.1‐mediated resistance in Arabidopsis against powdery mildew. We isolated and characterized Arabidopsis b7‐6 mutant. A point mutation in b7‐6 at the At5g12380 locus resulted in an amino acid substitution in ANNEXIN 8 (AtANN8). Loss‐of‐function or RNA‐silencing of AtANN8 led to enhanced expression of RPW8.1, RPW8.1‐dependent necrotic lesions in leaves, and defense against powdery mildew. Conversely, over‐expression of AtANN8 compromised RPW8.1‐mediated disease resistance and cell death. Interestingly, the mutation in AtANN8 enhanced RPW8.1‐triggered H₂O₂. In addition, mutation in AtANN8 led to hypersensitivity to salt stress. Together, our data indicate that AtANN8 is involved in multiple stress signaling pathways and negatively regulates RPW8.1‐mediated resistance against powdery mildew and cell death, thus linking ANNEXIN's function with plant immunity.

中文翻译：

ANNEXIN 8 负调控 RPW8.1 介导的拟南芥细胞死亡和抗病性

广谱抗性调控研究是植物生物学的一个活跃领域。抗白粉病 8.1 ( RPW8.1 ) 是少数广谱抗性基因之一，可触发过敏反应 (HR) 以限制多种病原体感染。为了解决 RPW8.1 信号传导如何调节的问题，我们进行了遗传筛选并试图确定增强 RPW8.1 介导的 HR 的突变。在这里，我们提供了将膜联蛋白与拟南芥中RPW8.1 介导的抗白粉病抗性联系起来的证据。我们分离并表征了拟南芥 b7-6突变体。b7-6的At5g12380点突变位点导致 ANNEXIN 8 (AtANN8) 中的氨基酸取代。AtANN8的功能丧失或 RNA 沉默导致RPW8.1 的表达增强，RPW8.1 依赖的叶片坏死病变和对白粉病的防御。相反，AtANN8 的过度表达会损害 RPW8.1 介导的抗病性和细胞死亡。有趣的是，AtANN8 中的突变增强了 RPW8.1 触发的 H ₂ O ₂。此外，AtANN8 中的突变导致对盐胁迫的超敏反应。总之，我们的数据表明 AtANN8 参与多种胁迫信号通路并负调节 RPW8.1 介导的对白粉病和细胞死亡的抗性，从而将 ANNEXIN 的功能与植物免疫联系起来。

更新日期：2020-10-19

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文本刊介绍/投稿指南11