Analysis of the organization and expression patterns of the convergent Pseudomonas aeruginosa lasR/rsaL gene pair uncovers mutual influence,Molecular Microbiology

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Analysis of the organization and expression patterns of the convergent Pseudomonas aeruginosa lasR/rsaL gene pair uncovers mutual influence
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-10-19 , DOI: 10.1111/mmi.14628
Silvia Schinner _{1,

2} , Matthias Preusse _{1,

2} , Christopher Kesthely ₂ , Susanne Häussler _{1,

2,

3,

4}

Affiliation

The two adjacent genes encoding the major Pseudomonas aeruginosa quorum‐sensing regulator, LasR, and its opponent, RsaL, overlap in their coding 3′ ends and produce mRNA transcripts with long untranslated 3′ ends that overlap with the sense transcripts of the gene on the opposing DNA strand. In this study, we evaluated whether the overlapping genes are involved in mutual regulatory events and studied interference by natural antisense transcripts. We introduced various gene expression constructs into a P. aeruginosa PA14 lasR/rsaL double deletion mutant, and found that although complementary RNA is produced, this does not interfere with the sense gene expression levels of lasR and rsaL and does not have functional consequences on down‐stream gene regulation. Nevertheless, expression of lasR, but not of rsaL, was shown to be enhanced if transcription was terminated at the end of the respective gene so that no overlapping transcription was allowed. Our data indicate that the natural organization with a partial overlap at the 3′ ends of the lasR/rsaL genes gives rise to a system of checks and balances to prevent dominant and unilateral control by LasR over the RsaL transcriptional regulator of opposing function.

中文翻译：

聚合铜绿假单胞菌 lasR/rsaL 基因对的组织和表达模式分析揭示了相互影响

编码主要铜绿假单胞菌群体感应调节因子 LasR 及其对手 RsaL的两个相邻基因在其编码 3' 末端重叠，并产生具有长未翻译 3' 末端的 mRNA 转录本，这些转录本与该基因的有义转录本重叠反向 DNA 链。在这项研究中，我们评估了重叠基因是否参与相互调节事件并研究了天然反义转录物的干扰。我们将各种基因表达构建体引入到铜绿假单胞菌PA14 lasR/rsaL双缺失突变体中，发现虽然产生了互补 RNA，但这不会干扰lasR和rsaL的正义基因表达水平并且对下游基因调控没有功能影响。然而，如果在相应基因的末端终止转录，则显示lasR 的表达增强，而不是rsaL 的表达增强，从而不允许重叠转录。我们的数据表明，在lasR/rsaL基因的 3' 端部分重叠的自然组织产生了一个制衡系统，以防止 LasR 对相反功能的 RsaL 转录调节因子进行显性和单方面控制。

更新日期：2020-10-19

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