Interactions between tissues such as epicardial adipose (EAT), and myocardial tissues is important in the pathogenesis of heart failure. Changes in adipose tissues in obesity or diabetes impair preadipocyte differentiation. Furthermore, proinflammatory cytokine secretion is higher in preadipocytes than in mature adipocytes in diabetes and obesity. However, how undifferentiated cells committed to the adipose lineage directly influence cardiomyocytes is not yet understood. We used human‐derived dedifferentiated fat (DFAT) cells as models of undifferentiated cells committed to an adipose lineage. Here, we evaluated the effects of soluble factor interactions in indirect cocultures of DFAT cells and induced pluripotent stem cell‐derived cardiomyocytes.

中文翻译：

---

去分化脂肪细胞释放的可溶性因子降低iPS细胞衍生心肌细胞的功能活性

心外膜脂肪 (EAT) 等组织与心肌组织之间的相互作用在心力衰竭的发病机制中很重要。肥胖或糖尿病中脂肪组织的变化会损害前脂肪细胞的分化。此外，在糖尿病和肥胖症中，前脂肪细胞的促炎细胞因子分泌高于成熟脂肪细胞。然而，尚未了解致力于脂肪谱系的未分化细胞如何直接影响心肌细胞。我们使用人源去分化脂肪 (DFAT) 细胞作为脂肪谱系未分化细胞的模型。在这里，我们评估了可溶性因子相互作用在 DFAT 细胞和诱导多能干细胞衍生的心肌细胞的间接共培养中的影响。