Evidences indicate that the decline of female fertility is becoming a common problem over the past few decades. Environmental exposure of Bisphenol A (BPA) has been considered as an endocrine‐disrupting chemical deleteriously affecting human reproductions. To better understand the effect of BPA exposure on human ovarian granulosa cells, we treated human ovarian granulosa cell line (KGN) with increasing concentrations (0.1, 1, 10, and 100 μM) of BPA for 24 hr. About 0.1, 1, and 10 μM BPA did not significantly affect the viability of KGN while 100 μM of BPA caused a statistically significant decrease in the viability of KGN. Treatment KGN with 10 μM BPA resulted in a significant decrease in progesterone biosynthesis. The treatment also significantly increased the expression of ATP binding cassette subfamily A member 1 (ABCA1) and steroidogenic acute regulatory protein (STAR). In the current study, exposure to BPA could lead to decreased progesterone production probably through the upregulation of ABCA1 in human granulosa cells.

中文翻译：

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双酚A降低人卵巢颗粒细胞中的孕酮合成

有证据表明，在过去的几十年里，女性生育能力的下降正在成为一个普遍的问题。双酚 A (BPA) 的环境暴露被认为是一种内分泌干扰化学物质，对人类生殖产生有害影响。为了更好地了解 BPA 暴露对人卵巢颗粒细胞的影响，我们用浓度增加（0.1、1、10 和 100 μM）的 BPA 处理人卵巢颗粒细胞系 (KGN) 24 小时。大约 0.1、1 和 10 μM BPA 不会显着影响 KGN 的生存力，而 100 μM 的 BPA 导致 KGN 生存力的统计学显着下降。用 10 μM BPA 处理 KGN 导致孕酮生物合成显着减少。该治疗还显着增加了 ATP 结合盒亚家族 A 成员 1 (ABCA1) 和类固醇生成急性调节蛋白 (STAR) 的表达。在目前的研究中，暴露于 BPA 可能通过上调人颗粒细胞中的 ABCA1 导致黄体酮产生减少。